Expression of NOS and HIF-1αin human colorectal carcinoma and implication in tumor angiogenesis

作     者：Jian-Xian Yu Lin Cui Qi-Yi Zhang Hua Chen Ping Ji Hong-Jun Wei Hai-yan Ma 

作者机构：Department of Pathology Qingdao Municipal Hospital Qingdao266011 Shandong Province China Department of Internal Medicine Qingdao Municipal Hospital Qingdao 266011 Shandong Province China Department of Pediatrics Qingdao MunicipalHospital Qingdao 266011 Shandong Province China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第29期

页      面：4660-4664页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Science Department of Qingdao City  No.03-1-NY-14-2 

主　　题：Nitric oxide synthase, HIF-1α Colorectalcarcinoma, Angiogenesis 

摘      要：AIM: To study CD34, CD105, inducible nitric oxide synthase (iNOS), endogenous nitric oxide synthase (eNOS), and hypoxia-inducible factor 1 (HIF-1)αexpression in human colorectal carcinomas. METHODS: The tissue microarrays (TMAs) were made up of 80 cases of colorectal carcinoma and 80 cases of non-neoplasm colorectal mucosa. The expression of CD34, CD105, NOS and HIF-1αwas detected by immunohistochemistry (S-P). RESULTS: iNOS and HIF-1αexpression in colorectal carcinoma was significantly higher than in non-neoplasm colorectal mucosa (X2 = 43.166, P 0.01; X2 = 10.4278, P 0.01); eNOS expression in colorectal carcinoma was significantly lower than in non-neoplasm colorectal mucosa (X2 = 11.354, P 0.01). The expression of iNOS correlated with differentiation (X2 = 18.141, P 0.01), invasive depth (X2 = 4.748, P 0.01), and Micro vessel density (MVD) (t = 2.327, P 0.05). The expression of HIF-1αwas correlated with infiltrating depth (X2 = 4.397, P 0.05), Duke s staging (X2= 4.255, P 0.05), and MVD (t = 2.272, P 0.05). No correlation was found in eNOS expression. CONCLUSION: Over-expression of iNOS and HIF-1αin colorectal carcinoma is correlated with the biological character MVD.