Identifi cation and characterization of a novel tetrapeptide from enzymatic hydrolysates of Baijiu byproduct

作     者：Qiang Wu Changqing Zhong Guirong Zeng Xu Zhang Liping Xiang Chang Wan Yougui Yu Qiang Wu;Changqing Zhong;Guirong Zeng;Xu Zhang;Liping Xiang;Chang Wan;Yougui Yu

作者机构：College of Food and Chemical EngineeringShaoyang UniversityShaoyang 422000China Department of Cardiovascular MedicineHunan Provincial People's HospitalChangsha 410005China Hunan Experimental Animal CenterHunan Drug Safety Evaluation and Research CenterLiuyang 410331China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第6期

页      面：1641-1649页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程] 

基　　金：sponsored by the Outstanding Youth Project of Hunan Education Department(19B505) Changsha City Science and Technology Plan Project(kq2004113) the 2020 Natural Science Foundation of Hunan Province of China(2020JJ8061) the 2020 Science and Technology Innovation Project of Hunan Province of China(2020SK50921) the Undergraduate Inquiry Learning and Innovative Experimental Project of Hunan Education Department(2019-1886) 

主　　题：Baijiu Distilled spent grain ACE inhibitory peptide Inhibition mechanism 

摘      要：In order to prepare angiotensin I-converting enzyme(ACE)inhibitory peptides,distilled spent grains of Chinese strong-flavor Baijiu were hydrolyzed by alcalase followed by papain under optimized conditions.A superior ACE inhibitory peptide was separated and purifi ed by ultrafi ltration and high-performance liquid chromatography(HPLC),and its amino acid sequence was further identified as Gln-Gly-Val-Pro(QGVP)by electrospray mass spectrometry(ESI-MS).QGVP formed 6 hydrogen bonds with the active site of ACE,which is responsible for reducingα-helix structure content of ACE causing subsequent inactivation.M oreover,it showed no significant cytotoxicity toward human umbilical vein endothelial cells(HUVECs),a nd signifi cantly i nduced phosphorylation of endothelial nitric oxide synthase(p-e NOS)and decreased endothelin 1(END1)expression in angiotensin I(Ang I)-treated HUVECs,demonstrating the potential antihypertensive *** peptide QGVP hydrolyzed from distilled spent grain proteins of Chinese strong-fl avor Baijiu was expected to be used as a food ingredient to prevent or co-treat hypertension with other chemical drugs.