APOPTOSIS AND TAXOL PRODUCTION IN SUSPENSION CULTURES OF Taxus ***

作     者：Yuan Y. J., Ma L Y., Ge Z. Q.,and Miao Z. Q. Zeng A.P. (Department of biochemical engineering, Tianjin University, Tianjin 300072, China) (Biochemical Engineering Division, GBF,D-38124 Breunschweig, Germany) 

出 版 物：《化工学报》 (CIESC Journal)

年 卷 期：2000年第51卷第S1期

页      面：43-46页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：apoptosis Taxol suspension culture Taxus spp. 

摘      要：The hallmark of apoptosis, in suspension cultures of Taxus spp. cells induced by fungal extractive or by abiotic means, was studied by total DNA agarose gel electrophoresis and in situ end-labeling. The cleavage of nuclear DNA (nDNA) into oligonucleosomal fragments(DNA laddering) was a characteristic of apoptosis, which involved cell shrinkage, condensation of cytoplasm and tracheary elements differentiation. Terminal deoxynucleotidy transferase-mediated dUTP nick end in situ labeling (TUNEL) assay of Taxus spp. cells showed that fungal extractive or abiotic elicltors (Ce4+, Taxol, H2O2) induced TUNEL positive. Also, the increase of the apoptotic cell ratio was accompanied by the increase of secondary metabolites (especially Taxol). These results suggest that apoptosis may have some coincidence with biosynthesis of Taxol. The implication of apoptosis for the production of secondary metabolites in plant cell cultures is discussed.