A biosensing system employing nanowell microelectrode arrays to record the intracellular potential of a single cardiomyocyte

作     者：Yuting Xiang Haitao Liu Wenjian Yang Zhongyuan xu Yue Wu Zhajian Tang Zhjing Zhu Zhiyong Zeng Depeng Wang Tianxing Wang Ning Hu Diming Zhang 

作者机构：Department of Obstetrics and GynecologyAffiliated Dongguan People's HospitalSouthern Medical UniversityDongguan 523058China Research Center for Inelligent Sensing SystemsZhejiang LaboratoryHangzhou 311100China Research Center for Humanoid SensingZhejiang LaboratoryHangzhou 311100China Key Laboratory of Novel Target and Drug Study for Neural Repair of Zhejiang ProvinceSchool of MedicineSchool of Computer&Computing ScienceZhejiang University City CollegeHangzhou 310015China School of Brain Science and Brain MedicineZhejiang UniversityHangzhou 310058China School of AutomationNanjing University of Science and TechnologyNanjing 210094China College of Energy and Power EngineeringNanjing University of Aeronautics and AstronauticsNanjing 210016China E-Link Care Meditech Co.LtdHangzhou 310011China ZJU-Hangzhou Global Scientific and Technological Innovation CenterDepartment of ChemitryZhejiang UniversityHangzhou 310058China 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2022年第8卷第3期

页      面：185-198页

核心收录：

学科分类：081704[工学-应用化学] 08[工学] 0817[工学-化学工程与技术] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the Center-initiated Research Project of Zheijiang Lab(Grant No.2021MHOALO1) the Startup Grant from Zhejang Lab(Grant No.113010-PI2108) 

主　　题：arrays potential record 

摘      要：Electrophysiological recording is a widely used method to investigate cardiovascular pathology,pharmacology and developmental *** arrays record the electrical potential of cells in a minimally invasive and highthroughput ***,commonly used microelectrode arays primarily employ planar microelectrodes and cannot work in applications that require a recording of the intracellular action potential of a single *** this study,we proposed a novel measuring method that is able to record the intracellular action potential of a single cardiomyocyte by using a nanowell patterned microelectrode array(NWMEA).The NWMEA consists of five nanoscale wells at the center of each circular planar *** pulse electroporation was applied to the NWMEA to penetrate the cardiomyocyte membrane,and the intracellular action potential was continuously *** intracellular potential recording of cardiomyocytes by the NWMEA measured a potential signal with a higher quality(213.76±25.85%),reduced noise root-mean-square(~33%),and higher signal-to-noise ratio(254.36±12.61%)when compared to those of the extracellular *** to previously reported nanopillar microelectrodes,the NWMEA could ensure single cell electroporation and acquire high-quality action potential of cardiomyocytes with reduced fabrication *** NWMEA-based biosensing system is a promising tool to record the intracellular action potential of a single cell to broaden the usage of microelectrode arrays in electrophysiological investigation.