Directed yeast genome evolution by controlled introduction of trans-chromosomic structural variations

作     者：Bin Jia Jin Jin Mingzhe Han Bingzhi Li Yingjin Yuan 

作者机构：Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第9期

页      面：1703-1717页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the Ministry of Science and Technology,the National Key Research and Development Program of China(2021YFC2100800) the National Natural Science Foundation of China(31800719,31861143017,21621004) 

主　　题：directed genome evolution structural variations SCRaMbLE synthetic genome synthetic biology 

摘      要：Naturally occurring structural variations(SVs)are a considerable source of genomic variation that can reshape the 3D architecture of *** methods aimed at introducing the complex SVs and their related molecular mechanisms have remained *** this study,an SV-prone yeast strain was developed using Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution(SCRaMbLE)technology with two synthetic chromosomes,namely synV and *** biosynthesis of astaxanthin is used as a readout and a proof of concept for the application of SVs in *** findings showed that complex SVs,including a pericentric inversion and a trans-chromosome translocation between synV and synX,resulted in two neo-chromosomes and a 2.7-fold yield of ***,genetic targets were mapped,which resulted in a higher astaxanthin yield,thus demonstrating the SVs’ability to reorganize genetic information along the *** rational design of trans-chromosome translocation and pericentric inversion enabled precise induction of these ***,this study provides an effective tool to not only accelerate the directed genome evolution but also to reveal the mechanistic insight of complex SVs for altering phenotypes.