Metformin alleviates LTA-induced inflammatory response through PPARγ/MAPK/NF-κB signaling pathway in bovine mammary epithelial cells

作     者：ABDELAZIZ ADAM IDRISS ARBAB CHUNQING YIN XUBIN LU YAN LIANG ISMAIL MOHAMED ABDALLA AMER ADAM IDRIS TIANLE XU YONGJIANG MAO ZHANGPING YANG 

作者机构：College of Animal Science and TechnologyYangzhou UniversityYangzhou225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety ofMinistry of Education of ChinaYangzhou UniversityYangzhou225009China Biomedical Research InstituteDarfur CollegeNyala63313Sudan Shandong Haiding husbandry Co.Ltd.Jinan250100China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第11期

页      面：2443-2454页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study was supported by the National Natural Science Foundation of China(Grant Nos.32102731 31872324) 

主　　题：Metformin LTA Primary bovine mammary epithelial cell Anti-inflammatory effect PPARγ Nuclear factor-κB Mitogen-activated protein kinase 

摘      要：Mastitis is a common inflammatory cow mammary infection;that causes significant economic loss in dairy *** the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs,our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase(MAPK)and nuclear factor kappa B(NF-κB)signaling pathways and activation of peroxisome proliferator-activated receptor-γ(PPARγ)in *** proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR).Immunoblotting and immunofluorescence analysis were conducted to evaluate the expression of target proteins in inflammatory and anti-inflammatory responses to metformin and ***,pbMECs were allowed to treat with the PPAR antagonist GW9662,and inflammatory markers were detected in the *** results showed that LTA concentration at 100μg/mL significantly stimulated the MAPK14,IL-6 and IL-1βmRNA expressions compared to the control cells(P0.05)in dose-dependent tests for *** suppressed the phosphorylation expressions of MAPK(ERK1/2,p38,and JNK)in LTA-stimulated *** also reduced the protein expression of NF-κB,interleukin-8(IL-8),interleukin-1β(IL-1β)and interleukin-6(IL-6)in pbMECs pretreated with *** administration activated PPARγphosphorylation by up-regulating the expression of PPARγin LTA-stimulated *** with GW9662 resulted in increased IL-6 expression,which was reversed by *** findings collectively indicated that metformin act to attenuate LTA-stimulated inflammatory response in pbMECs by suppressing MAPK and NF-κB activation via a mechanism partially dependent on PPARγ*** results suggested that metformin could function as an anti-inflammatory drug in the treatment of mastitis.