Unveiling Cellular Internalization Dynamics of Single Gold Nanorods by Tracking Their Orientational and Translational Motions

作     者：Dong Xu Yi Du Feng Ge Yan He Edward S.Yeung 

作者机构：National Engineering Laboratory for Rice and By-Products Further ProcessingCollege of Food Science and EngineeringCentral South University of Forestry&TechnologyChangsha 410004 State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyHunan UniversityChangsha 410082 Department of ChemistryKey Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education)Tsinghua UniversityBeijing 100084 Department of ChemistryIowa State UniversityAmesIA 50011 

出 版 物：《CCS Chemistry》 (中国化学会会刊（英文）)

年 卷 期：2022年第4卷第4期

页      面：1295-1304页

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(grant nos.21127009,21221003,and 21425519) the Training Program for Excellent Young Innovators of Changsha(grant no.kq1905061) the Natural Science Foundation of Hunan Province,China(grant no.020RC3042) 

主　　题：orientation distribution pattern nanoparticle cellular internalization orientational and translational motion single nanoparticle tracking 

摘      要：Single nanoparticle tracking(SPT)is a unique and powerful tool to investigate the interaction between nanoparticles and cells,which is of considerable importance for nanotechnology applications in biomedical fields and in-depth understanding of biological ***,previous work typically focused on translations of single nanoparticles while they undergo both translational and rotational *** this study,we obtained both the translational and rotational dynamics of single gold nanorods during their cellular internalization process using dual-channel polarization *** particular,the azimuth and polar angles were integrated into a polar coordinate systemto obtain three general orientation distribution patterns,found to have a close relationship with the nanoparticle cellular internalization process and time-dependent ***,the patterns accompanied by trajectories,translational and rotational coefficients,the azimuth and polar angles,and other parameters provided a wealth of knowledge on the nanoparticle cellular internalization dynamics with unprecedented *** observed that the gold nanorods could initially assume a tip-first quick rotation state with partially restricted orientations,then change to a strongly confined near-vertical insertion state with slight angular fluctuations,and eventually transform into a random and fast rotation *** methodology opens up a new avenue for a detailed understanding of biological processes.