Caging-Decaging Strategies to Realize Spatiotemporal Control of DNAzyme Activity for Biosensing and Bioimaging
作者机构：Institute of Chemical Biology and NanomedicineState Key Laboratory of Chemo/Biosensing and ChemometricsHunan UniversityChangsha410082P.R.China College of Chemistry and Chemical EngineeringHunan UniversityChangsha410082P.R.China College of BiologyHunan UniversityChangsha410082P.R.China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2022年第38卷第4期
页 面：902-911页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0703[理学-化学] 0836[工学-生物工程]
基 金：supported by the National Natural Science Foundation of China(No.21877032) the Hunan Province Talented Young Scientists Program,China(Nos.2019RS2021,2019RS2023) the Open Research Fund Program of the State Key Laboratory of Analytical Chemistry for Life Sciences,China(No.SKLACLS2102) the Scientific Research Program of Shenzhen Institute of Synthetic Biology,China(No.DWKF20210005) the Fundamental Research Funds for the Central Universities,China
主 题：RNA-cleaving DNAzyme Caging-decaging strategy Spatiotemporal control Biosensing Bioimaging
摘 要：DNAzymes with RNA-cleaving activity have been widely used as biosensing and bioimaging tools for detection of metal *** the achievements,DNAzyme-based biosensors sometime suffer from false positive signals and unexpected off-target turn-on in biological environments,which are likely due to the unstable nature of the RNA *** to control DNAzyme activity in order to improve the sensing performance remain a significant *** meet the challenge,there is growing interest to develop synthetic strategies that can cage native DNAzyme under undesired conditions and reactivate it in target environment in order to function in a controlled manner.A variety of caging-decaging strategies have been developed to realize spatiotemporal control of the DNAzyme activity,improving its specificity and sensitivity as well as extending its application *** this review,we focus on strategies to regulate the catalytic activity of DNAzyme,highlight the nucleic acid modification chemistries,and summarize three strategies to cage DNAzyme *** of using caged DNAzyme for bio-applications have also been reviewed in ***,we provide our perspectives on the potential challenges and opportunities of this emerging research topic that could advance the DNAzyme field.
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