Miniaturized structured illumination microscopy with diffractive optics

作     者：GUOXUAN LIU NING XU HUAIDONG YANG QIAOFENG TAN GUOFAN JIN GUOXUAN LIU;NING XU;HUAIDONG YANG;QIAOFENG TAN;GUOFAN JIN

作者机构：State Key Laboratory of Precision Measurement Technology and InstrumentsDepartment of Precision InstrumentTsinghua UniversityBeijing 100084China 

出 版 物：《Photonics Research》 (光子学研究（英文版）)

年 卷 期：2022年第10卷第5期

页      面：1317-1324页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 080203[工学-机械设计及理论] 0802[工学-机械工程] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：National Natural Science Foundation of China(62075112) 

主　　题：illumination optics system 

摘      要：Structured illumination microscopy(SIM)is an advanced microscope system that provides superresolution capability with excellent imaging speed,which has become a practical tool for live-cell ***,the bulky size is blocking the application of SIM in wider study fields and ***,we developed a miniaturized SIM(Mini SIM)system that provided periodic illumination using a diffractive optical element(DOE)for the first *** optimized phase-only DOE generated the two-dimensional sinusoidal illumination by optical Fourier transform with an illuminating objective lens,which substantially simplified and miniaturized the illumination *** built up a Mini SIM prototype and demonstrated lateral superresolution imaging of fluorescence beads and A549 cell *** proposed Mini SIM greatly simplifies the experimental setup and may lead to important applications in bio-imaging.