Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection ofβ-lactoglobulin allergen in milk products

作     者：Mingfei Pan Liping Hong Jingying Yang Xiaoqian Xie Kaixin Liu Shuo Wang Mingfei Pan;Liping Hong;Jingying Yang;Xiaoqian Xie;Kaixin Liu;Shuo Wang

作者机构：Key Laboratory of Food Nutrition and SafetyMinistry of Education of ChinaTianjin University of Science and TechnologyTianjin 300457China State Key Laboratory of Food Nutrition and SafetyTianjin University of Science&TechnologyTianjin 300457China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第5期

页      面：1315-1321页

核心收录：

学科分类：080903[工学-微电子学与固体电子学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 083203[工学-农产品加工及贮藏工程] 

基　　金：supported by the National Natural Science Foundation of China(31972147) Project of Tianjin Science and Technology Plan(19PTSYJC00050) the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology(SKLFNS-KF-202011) Project program of Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin Key Laboratory of Food Nutrition and Safety,China(JYB202002) 

主　　题：β-Lactoglobulin allergen Quartz crystal microbalance Immunochip Milk products 

摘      要：In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk *** parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in *** optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/***,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten *** the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.