Influence of biological sex and exercise on murine cardiac metabolism

作     者：Kyle Fulghum Helen E.Collins Steven P.Jones Bradford G.Hill Kyle Fulghum;Helen E.Collins;Steven P.Jones;Bradford G.Hill

作者机构：Diabetes and Obesity CenterDepartment of MedicineDivision of Environmental MedicineChristina Lee Brown Envirome InstituteUniversity of LouisvilleLouisvilleKY 40202USA Department of PhysiologyUniversity of LouisvilleLouisvilleKY 40202USA 

出 版 物：《Journal of Sport and Health Science》 (运动与健康科学（英文）)

年 卷 期：2022年第11卷第4期

页      面：479-494页

核心收录：

学科分类：0403[教育学-体育学] 040302[教育学-运动人体科学(可授教育学、理学、医学学位)] 04[教育学] 

基　　金：Supported in part by grants from the NIH (HL154663, HL147844,HL130174,HL078825,GM127607) supported by the Jewish Heritage Fund for Excellence 

主　　题：Ketone bodies Metabolomics Mitochondria Physical activity Sex difference 

摘      要：Although the structural and functional effects of exercise on the heart are well established,the metabolic changes that occur in the heart during and after exercise remain *** this study,we used metabolomics to assess time-dependent changes in the murine cardiac metabolome following 1 session of treadmill *** the exercise bout,we also recorded blood lactate,glucose,and ketone body levels and measured cardiac mitochondrial *** both male and female mice,moderate-and high-intensity exercise acutely increased blood lactate *** both sexes,low-and moderate-intensity exercise augmented circulating 3-hydroxybutryrate levels immediately after the exercise bout;however,only in female mice did high-intensity exercise increase 3-hydroxybutyrate levels,with significant increases occurring 1 h after the exercise *** metabolomics analyses of sedentary female and male hearts suggest considerable sex-dependent differences in basal cardiac metabolite levels,with female hearts characterized by higher levels of pantothenate,pyridoxamine,homoarginine,tryptophan,and several glycerophospholipid and sphingomyelin species and lower levels of numerous metabolites,including acetyl coenzyme A,glucuronate,gulonate,hydroxyproline,prolyl-hydroxyproline,carnosine,anserine,and carnitinylated and glycinated species,as compared with male *** after a bout of treadmill exercise,both male and female hearts had higher levels of corticosterone;however,female mice showed more extensive exercise-induced changes in the cardiac metabolome,characterized by significant,time-dependent changes in amino acids(e.g.,serine,alanine,tyrosine,tryptophan,branched-chain amino acids)and the ketone body *** from experiments using isolated cardiac mitochondria suggest that high-intensity treadmill exercise does not acutely affect respiration or mitochondrial coupling;however,female cardiac mitochondria demonstrate generally higher adenosi