Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

作     者：Ching-Chung Liang Steven W Shaw Yung-Hsin Huang Tsong-Hai Lee 

作者机构：Female Urology SectionDepartment of Obstetrics and GynecologyLinkou Chang Gung Memorial HospitalCollege of MedicineChang Gung UniversityTaoyuan 333Taiwan Division of ObstetricsDepartment of Obstetrics and GynecologyTaipei Chang Gung Memorial HospitalTaipei 105Taiwan Prenatal Cell and Gene Therapy GroupInstitute for Women’s HealthUniversity College LondonLondon WC1E 6BTUnited Kingdom Stroke Center and Department of NeurologyLinkou Chang Gung Memorial HospitalCollege of MedicineChang Gung UniversityTaoyuan 333Taiwan 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2022年第14卷第5期

页      面：330-346页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by the Linkou Chang Gung Memorial Hospital grants,No. CMRPG3J0951 and No. CMRPG3H1041-2 Ministry of Science and Technology Taiwan grants,No. MOST 107-2314-B-182A-101 and No. MOST 109-2314-B-182A-084 

主　　题：Amniotic fluid Bladder dysfunction Diabetes Insulin Stem cells 

摘      要：BACKGROUND Diabetes mellitus(DM)is a serious and growing global health *** is estimated that 80%of diabetic patients have micturition problems such as poor emptying,urinary incontinence,urgency,and urgency *** with diabetic bladder dysfunction are often resistant to currently available *** is necessary to develop new and effective treatment *** To examine the therapeutic effect of human amniotic fluid stem cells(hAFSCs)therapy on bladder dysfunction in a type 2 diabetic rat *** Sixty female Sprague-Dawley rats were divided into five groups:Group 1,normal-diet control(control);group 2,high-fat diet(HFD);group 3,HFD plus streptozotocin-induced DM(DM);group 4,DM plus insulin treatment(DM+insulin);group 5,DM plus hAFSCs injection via tail vein(DM+hAFSCs).Conscious cystometric studies were done at 4 and 12 wk after insulin or hAFSCs treatment to measure peak voiding pressure,voided volume,intercontraction interval,bladder capacity,and residual *** and/or mRNA expression of muscarinic receptors,nerve growth factor(NGF),and sensory nerve markers in the bladder and insulin,MafA,and pancreatic-duodenal homeobox-1(PDX-1)in pancreatic beta cells were *** Compared with DM rats,insulin but not hAFSCs treatment could reduce the bladder weight and improve the voided volume,intercontraction interval,bladder capacity,and residual volume(P0.05).The immunoreactivities and mRNA expression of M2-and M3-muscarinic receptors(M2 and M3)were increased mainly at 4 wk(P0.05),while the number of beta cells in islets and the immunoreactivities and/or mRNA of NGF,calcitonin gene-related peptide(CGRP),substance P,insulin,MafA,and PDX-1 were decreased in DM rats(P0.05).However,insulin and hAFSCs treatment could help to regain the expression of M2,M3,NGF,CGRP,substance P,MafA