OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak

作     者：Lihan Wang Jie Chen Yuqin Zhao Shiping Wang Meng Yuan Lihan Wang;Jie Chen;Yuqin Zhao;Shiping Wang;Meng Yuan

作者机构：National Key Laboratory of Crop Genetic ImprovementNational Center of Plant Gene Research(Wuhan)Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第5期

页      面：1116-1130页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported by grants from the National Natural Science Foundation of China(31821005,31871946,32172421) the National Science Foundation of Hubei Province(2020CFA058)。 

主　　题：OsLIC OsMAPK6 OsWRKY30 rice Xanthomonas oryzae 

摘      要：Rice OsLIC encoding a CCCH zinc finger transcription factor plays an important role in immunity.However,the immune signaling pathways that OsLIC-involved and the underlying mechanisms that OsLIC-conferred resistance against pathogens are largely unclear.Here,we show that OsLIC,as a substrate for Os MAPK6,negatively regulates resistance to Xanthomonas oryzae pv.oryzae(Xoo)and X.oryzae pv.oryzicola(Xoc)by directly suppressing OsWRKY30 transcription.Biochemical assays showed that OsLIC bound to OsWRKY30 promoter and suppressed its transcription.Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc,knocking out OsWRKY30 in the oslic mutants attenuated the resistance against bacterial pathogens.Os MAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity,therefore,overexpression of OsLIC partially suppressed Os MAPK6-mediated rice resistance.In addition,both Os MAPK6-phosphorylated activation of OsLIC and phosphorylation-mimic OsLIC_(5D) had reduced DNA-binding activity towards OsWRKY30 promoter,thereby promoting OsWRKY30 transcription.Collectively,these results reveal that Os MAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription,suggesting that Os MAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.