Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis:In vitro and in vivo study
作者机构：Department of Biological SciencesFaculty of ScienceKing Faisal UniversityP.O.Box 380Al-Ahsa 31982Saudi Arabia Department of Botany and MicrobiologyFaculty of ScienceCairo UniversityCairo 12613Egypt Department of ZoologyFaculty of ScienceAin Shams UniversityCairo11566Egypt
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2022年第12卷第6期
页 面：243-252页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学]
基 金：the Deanship of Scientific Research Vice Presidency for Graduate Studies and Scientific Research King Faisal University Saudi Arabia [Project No. GRANT93 (170061)]
主 题：Aspergillus fumigatus Antifungal Invasive pulmonary aspergillosis Calotropis gigantea Amphotericin B Mice
摘 要：Objective:To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in ***:GC/MS was used for analysis of active constituents of Calotropis gigantea *** germination assay and broth micro-dilution method were used to determine antifungal potential of Calotropis gigantea/amphotericin B against Aspergillus *** mice were randomly assigned into 5 groups:group 1 was neutropenic(control);group 2 was infected with Aspergillus fumigatus;group 3 was infected with Aspergillus fumigatus,and treated with Calotropis gigantea extract;group 4 was infected with Aspergillus fumigatus and treated with amphotericin B;group 5 was infected with Aspergillus fumigatus and treated with both Calotropis gigantea extract and amphotericin *** lung tissues were histopathologically *** burden and gliotoxin concentration were evaluated in lung ***,superoxide dismutase,and malondialdehyde content were determined in lung ***,tumor necrosis factor-alpha,interleukin-1,and interleukin-17 were also estimated by the sandwich enzyme-linked immuno-sorbent ***:Calotropis gigantea/amphotericin B had a minimum inhibitory concentration and minimum fungicidal concentration of 80 and 160μg/mL,respectively,for Aspergillus ***,Calotropis gigantea/amphotericin B significantly reduced lung fungal burden by 72.95%and inhibited production of gliotoxin in lung tissues from 6320 to 1350μg/g *** gigantea/amphotericin B reduced the oxidative stress of the lung via elevating the activity of antioxidant enzymes and decreasing the levels of lipid *** activity and the production of pro-inflammatory cytokines were also significantly *** electron microscopy revealed deteriorations in the hyphae ultrastructure in Calotropis gigantea/amphotericin B trea
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