Tetrahedral framework nucleic acids regulate osteogenic differentiation potential of osteoporotic adipose-derived stem cells

作     者：Tianyu Chen Dexuan Xiao Yanjing Li Sirong Shi Xiao Yang Shuanglin Peng Bin Guo Xiaoxiao Cai Tianyu Chen;Dexuan Xiao;Yanjing Li;Sirong Shi;Xiao Yang;Shuanglin Peng;Bin Guo;Xiaoxiao Cai

作者机构：State Key Laboratory of Oral DiseasesNational Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China Department of StomatologyFirst Medical Center of Chinese PLA General HospitalBeijing 100853China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第5期

页      面：2517-2521页

核心收录：

学科分类：1002[医学-临床医学] 07[理学] 070205[理学-凝聚态物理] 0703[理学-化学] 0702[理学-物理学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(No.2019YFA0110600) National Natural Science Founda-tion of China(Nos.82171006,81970986,81771125,82001432) China Postdoctoral Science Foundation(Nos.2020TQ0213,2020M683319) West China Hospital Postdoctoral Science Foundation(No.2020HXBH104). 

主　　题：Osteoporosis Tetrahedral framework nucleic acid Adipose-derived stem cell Autologous stem-cell transplantation Osteogenic differentiation 

摘      要：Osteoporosis(OP)is a noncommunicable bone disease caused by a shift in the balance between os-teoblasts and osteoclasts,and can severely affect the health of elderly persons.Autologous stem-cell transplantation can improve reduced bone density and weakened fracture healing abilities in patients with OP.However,OP can adversely affect the osteogenesis and proliferation abilities of autologous adipose-derived stem cells(ASCs).Therefore,an effective drug is required to facilitate autologous ASCs to recover their osteogenic and proliferative potential.Tetrahedral framework nucleic acid(tFNA)is a new type of nanomaterial that has ability to regulate the biological behavior of cells effectively and en-hance the bioactivity of stem cells.In this study,we examine the effects of tFNAs on the osteogenic differentiation and proliferation abilities of ASCs in rats with OP.The results indicate that the 250 nmol/L tFNAs can considerably increase the expression of osteogenesis-related markers,effectively promote the proliferation and osteogenic differentiation of osteoporotic ASCs(OP-ASCs),and help them to regain their osteogenic and proliferative potential.In short,tFNAs can enable OP-ACSs to recover their osteogenic po-tential and promote their proliferation and,therefore,can play a key regulatory role in autologous ASC transplantation.