Clinical distribution and molecular profiling on postoperative colorectal cancer patients with different traditional Chinese medicine syndromes
作者机构：Department of Traditional Chinese MedicineHangzhou Jianggan District People’s HospitalHangzhouChina Department of Medical OncologySir Run Run Shaw HospitalCollege of MedicineZhejiang UniversityHangzhouChina Holistic Integrative Pharmacy Institutes and Comprehensive Cancer Diagnosis and Treatment Centerthe Affiliated Hospital of Hangzhou Normal UniversityCollege of MedicineHangzhou Normal UniversityHangzhouChina Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province and Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang ProvinceHangzhou Normal UniversityHangzhouChina
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2020年第5卷第1期
页 面：44-52页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This research was supported by grants from National Natural Science Foundation of China(grant No.81874380,81672932,81730108 and 81973635) Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars(grant No.LR18H160001) Zhejiang Province Science and Technology Project of TCM(grant No.2019ZZ016) Zhejiang Province Medical Science and Technology Project(grant No.2017RC007) Talent Project of Zhejiang Association for Science and Technology(grant No.2017YCGC002) Key Project of Hangzhou Ministry of Science and Technology(grant No.20162013A07) Zhejiang Provincial Project for the Key Discipline of Traditional Chinese Medicine(grant No.2017-XK-A09) the Open Project Program of Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica(No.JKLPSE201807) the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
主 题：Traditional Chinese medicine Clinical distribution Molecular profiling Colorectal cancer Syndrome differentiation Pattern diagnosis
摘 要：Background:Traditional Chinese medicine(TCM)syndrome,also named syndrome,are comprehensive and integral analyses of clinical information which helps to guide different individualized treatment prescriptions.Methods:Thirty healthy controls and 80 colorectal cancer(CRC)patients(including 33 Spleen Qi Deficiency syndrome,23 Dampness Heat syndrome,17 Blood Stasis syndrome and 7 other syndrome)were enrolled into this study.Human mRNAs were extracted from peripheral blood mononuclear cells.The gene expression for CRC patients with different TCM syndrome was determined by microarray and qRT-PCR.Results:Spleen Qi Deficiency,Dampness Heat and Blood Stasis were the most common syndromes in CRC patients.There is a significant difference was found in mRNA expression levels(especially for PIK3CA,STAT3,SOX9 and KDM5C)among Spleen Qi Deficiency,Dampness Heat and Blood Stasis syndrome groups.The higher mRNA levels of JNK1,TP53,MLH1,MSH6,PMS2,SOCS3,TCF7L2,FAM123B,PSAP,FBXW7,SALL4 and the lower expression of inflammatory cytokine IL-6 were found in Spleen Qi Deficiency group but not other syndrome types.The higher mRNA levels of KRAS,MUC16,EGFR,GRASP65,PIK3CA,MAPK7,CD24,STAT3,SLC11A1,Bcl-2,TXNDC17 and some inflammatory cytokines(IL-6,IL-23,TNF-a,CXCR4)were found in Dampness Heat group but not other syndrome types.Blood Stasis syndrome showed higher expression of SOX9,MLH1,MSH6,KDM5C,PCDH11X,PSAP and SALL4,and lower mRNA levels of PIK3CA,CD24,STAT3,CXCR4,TXNDC17 and TP53.The CRC patients with Dampness Heat syndrome might have a poor prognosis than other syndrome types.Conclusion:The identification of syndrome conditions had different impacts on CRC prognosis,and which might be related with different mRNA expression levels.Some oncogenes and pro-inflammatory cytokines were highly expressed in Dampness Heat group but not other syndrome types,suggesting that the CRC patients with Dampness Heat syndrome might have a poor prognosis.Our results prelimitarily uncovered the molecular basis of syndrome differences in CRC prognosis,a better understanding for TCM treatment of CRC.
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