Optic nerve crush as a model of retinal ganglion cell degeneration
作者机构：Department of BiologyUniversity of PisaPisaItaly
出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)
年 卷 期：2022年第7卷第2期
页 面：27-32页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Axonal injury retrograde degeneration cell death
摘 要：Background:Axonal degeneration caused by damage to the optic nerve can result in a gradual death of retinal ganglion cells(RGC),leading to irreversible vision *** example of such diseases in humans includes optic nerve degeneration in *** is characterized by the progressive degeneration of the optic nerve and the loss of RGCs that can lead to loss of *** different animal models developed to mimic glaucomatous neurodegeneration,all result in RGC loss consequent optic nerve ***:The present article summarizes experimental procedures and analytical methodologies related to one experimental model of glaucoma induced by optic nerve crush(ONC).Point-by-point protocol is reported with a particular focus on the critical point for the realization of the ***,information on the electroretinogram procedure and the immunohistochemical detection of RGCs are described to evaluate the morpho-functional consequences of ***:Although the model of ONC is improperly assimilated to glaucoma,then the ONC model simulates most of the signaling responses consequent to RGC apoptosis as observed in models of experimental *** this respect,the ONC model may be essential to elucidate the cellular and molecular mechanisms of glaucomatous diseases and may help to develop novel neuroprotective therapies.
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