Microarray expression profile and functional analysis of circular RNAs in choroidal neovascularization

作     者：Jiancheng Huang Meng Chen Kai Xu Rongmei Zhou Shujie Zhang Chen Zhao Jiancheng Huang;Meng Chen;Kai Xu;Rongmei Zhou;Shujie Zhang;Chen Zhao

作者机构：Department of Ophthalmologythe First Affiliated Hospital of Nanjing Medical UniversityState Key Laboratory of Reproductive MedicineNanjingJiangsu 210029China Eye InstituteEye and ENT HospitalShanghai Medical CollegeFudan UniversityShanghai 200031China NHC Key Laboratory of Myopia(Fudan University)Key Laboratory of MyopiaChinese Academy of Medical Sciencesand Shanghai Key Laboratory of Visual Impairment and Restoration(Fudan University)Shanghai 200032China. 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2020年第34卷第1期

页      面：67-74页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81525006,81670864,and 81730025 to C.Z.) the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2018PT32019 to C.Z.)。 

主　　题：circRNAs microarray choroidal neovascularization 

摘      要：Choroidal neovascularization(CNV) is a leading cause of visual loss in age-related macular degeneration(AMD). However, the molecular mechanism for CNV progression is still unclear. This study aimed to identify CNV-related circular RNAs(circRNAs), a novel class of non-coding RNAs with diverse functions. A total of 117 circRNAs were differentially expressed in the murine CNV model by microarrays. Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis were performed to identify the functions of selected circRNAs. The host genes of these circRNAs were predicted to be targeted to neurogenesis(ontology: biological process), proteinaceous extracellular matrix(ECM)(ontology: cellular component), and binding(ontology: molecular function). Differentially expressed circRNAs-mediated regulatory networks were enriched in ECM receptor interaction. Most of the dysregulated circRNAs could potentially bind to five different mi RNAs by Target Scan and mi Randa. Specifically, circ_15752 was identified in this circRNAs pool which may facilitate vascular endothelial cell proliferation, migration, and tube formation, suggesting a critical role in endothelial angiogenesis. Our work suggests that dysregulated circRNAs may be involved in CNV pathogenesis and serve as potential biomarkers for CNV.