Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)

作     者：Wan-Nor Fitri Haron Wahid Putra Tengku Rinalfi Dana Raj Yawah Donny Latip Qayyum Ab Aziz Abdul Malek Wan-Nor Fitri;Haron Wahid;Putra Tengku Rinalfi;Dana Raj;Yawah Donny;Latip Qayyum;Ab Aziz Abdul Malek

作者机构：Department of Veterinary Clinical StudiesFaculty of Veterinary MedicineUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Research Centre for WildlifeFaculty of Veterinary MedicineUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Veterinary Pre-Clinical StudiesFaculty of Veterinary MedicineUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Sarang Buaya Pasir GudangAras 2 Menara AqabahJalan Bandar81700 Pasir GudangJohorMalaysia Wildlife Veterinary SectionEx-Situ Conservation DivisionDepartment of Wildlife and National Parks(PERHILITAN)Peninsular Malaysia 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2020年第9卷第2期

页      面：104-108页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 100101[医学-人体解剖与组织胚胎学] 0906[农学-兽医学] 0901[农学-作物学] 0902[农学-园艺学] 10[医学] 

主　　题：Spermatozoa Scanning electron microscopy Morphology Crocodile Cytoplasmic droplets 

摘      要：Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus digitally from the base of the penis was performed on four adult male crocodiles to facilitate the flow of semen to the sulcus. Semen was collected from all individuals for spermatozoa morphometry and ultrastructure study. Morphometry analysis was performed from eosin-nigrosin stained spermatozoa sample. Scanning electron microscopy was conducted to observe the surface ultrastructure of spermatozoa. Results: The morphology of crocodile spermatozoa was made up of acrosome, head, and tail which corresponded to (5.55±1.20) μm, (12.74±1.57) μm, and (70.67±4.40) μm, respectively. The total length of spermatozoa in estuarine crocodile was measured at (88.96±0.52) μm. The most common spermatozoa abnormalities found in the samples were cytoplasmic droplets. Conclusions: Normal morphology, morphometry of spermatozoa in estuarine crocodile which are vermiform shaped-head with a long tail along with questionably high cytoplasmic droplets count are described in this study.