The truncated form of fagellin (tFlic) provides the 2dCap subunit vaccine with better immunogenicity and protective efects in mice
作者机构：Department of Veterinary MedicineInstitute of Preventive Veterinary Medicine&Zhejiang Provincial Key Laboratory of Preventive Veterinary MedicineCollege of Animal SciencesZhejiang University866 Yuhangtang roadHangzhou 310058China
出 版 物：《Animal Diseases》 (动物疾病（英文）)
年 卷 期：2022年第2卷第2期
页 面：118-126页
基 金：This work was supported by the Key Laboratory of Veterinary Biological Products and Chemicals Ministry of Agriculture China Animal Husbandry Industry Co. Ltd.Project name:Novel Adjuvanted Chimeric Porcine Circovirus Type 2 CAP Virus-Like Particle Vaccine and project number:20201612(2020-2024)
主 题：Porcine circovirus type 2(PCV2) Virus-like particles(VLPs) Truncated form of fagellin(tFlic) Immunogenicity
摘 要：Porcine circovirus type 2(PCV2)is the main causative agent of porcine circovirus-associated diseases,and it causes substantial economic losses in the swine industry each *** is crucial to develop an efective vaccine against the circulating strain PCV2d,which is prone to substantial degrees of *** this study,a truncated form of fagellin(tFlic:85-111 aa)was inserted into the C-terminal sequence of 2dCap,and Western blotting results showed that recombinant Cap-tFlic VLPs were successfully *** electron microscopy(TEM)and dynamic light scattering(DLS)data indicated that purifed recombinant Cap-tFlic fusion proteins existed in the form of polymers and that tFlic could not afect the formation and internalization of *** Cap-tFlic VLPs induced the expression of antigen presentation-related factors(MHC-II and CD86)by bone marrow-derived dendritic cells(BM-DCs),and the expression of TLR5-related factors(TNF-α)was dramatically *** intramuscularly immunized with Cap-tFlic VLPs exhibited signifcantly higher levels of Cap-specifc antibodies and neutralizing antibodies than mice immunized with wild-type Cap *** data obtained in the current study indicate that Cap-tFlic may be a candidate for a subunit vaccine against PCV2 in the future.
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