Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory

作     者：Li SUN Peike GAO Yu LI Chao WANG Ning DING Junfeng CHEN Yuhao SONG Chunchen LIU Lun SONG Renjun WANG Li SUN;Peike GAO;Yu LI;Chao WANG;Ning DING;Junfeng CHEN;Yuhao SONG;Chunchen LIU;Lun SONG;Renjun WANG

作者机构：College of Life SciencesQufu Normal UniversityQufu 273165China Key Laboratory of Marine Biological Resources and EcologyLiaoning Ocean and Fisheries Science Research InstituteDalian 116023China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2022年第40卷第3期

页      面：1027-1038页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 0707[理学-海洋科学] 0815[工学-水利工程] 0713[理学-生态学] 

基　　金：Supported by the National Natural Science Foundation of China(Nos.31971503,31901188) the Shandong Provincial Agricultural Fine Species Project(No.2019LZGC020) the Jining Key Research and Development Project of Shandong Province(No.2019ZDGH019) the Shandong Provincial Natural Science Foundation(Nos.ZR2019BB040,ZR2020MC042) the Interdisciplinary Project of Qufu Normal University(No.XKJJC201903) the Key Research and Development Project of Liaoning Province(No.2018228004) the Revitalization Talents Program of Liaoning Province(No.XLYC1907109),the Shandong Provincial Key Research and Development Project(No.2018GSF117035) the Shandong Provincial Higher Educational Science and Technology Program(No.J17KA112)。 

主　　题：Karenia mikimotoi microbial community nutrient stimulation algicidal bacteria Pseudoalteromonas 

摘      要：Algicidal bacteria have been frequently isolated from algal blooming areas.However,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is limited.In this study,we investigated the composition of the microbial community coexisting with harmful alga Karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in microcosms.The microorganisms inhabiting the K.mikimotoi culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal cultivation.Both the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of K.mikimotoi and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas increased.Core operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the K.mikimotoi culture with or without treatments.Collectively,this study indicated that microbial community inhabiting K.mikimotoi culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of K.mikimotoi via nutrient stimulation or by adding isolated algicidal strains.