Efficacy of phospholipid complex of flavonoids from persimmon leaves on atherosclerosis, and possible mechanism

作     者：CHEN Jinpeng ZHANG Kexia LIU Yi JIN Song GAI Xiaohong REN Tao TIAN Chengwang CHEN Jinpeng;ZHANG Kexia;LIU Yi;JIN Song;GAI Xiaohong;REN Tao;TIAN Chengwang

作者机构：State Key Laboratory of Drug Delivery and PharmacokineticsTianjin 300301China Tianjin Key Laboratory of TCM quality markersTianjin 300301China Tianjin Institute of Pharmaceutical ResearchTianjin 300301China Tianjin Pharmaceutial Research Institute Co.Ltd.Tianjin 300462China Sinopharm Group Tianjin Co.Ltd.Tianjin 300040China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第3期

页      面：417-425页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by National Science and Technology Major Project of China(Research on Evaluation Technology of New Traditional Chinese Medicine Based on Big Data of Classical Prescription,No.2019ZX09201005) International Cooperation Project of Traditional Chinese Medicine(China-Germany International Cooperation in Innovative Research and Development of Traditional Chinese Medicine and Botanical Medicine,No.0610-2140NF020630) 

主　　题：flavonoids chromatography,high pressure liquid mass spectrometry phospholipids lipoproteins,LDL endothelial cells 

摘      要：OBJECTIVE:To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves(PLF-PC)on atherosclerosis,and to study its mechanism behind the ***:To clarify the constituents of the flavonoids from persimmon leaves(PLF),an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was *** enhance the anti-atherosclerotic effect of PLF,a newly emerging approach based on the combination of phospholipid complexation technique was ***-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy,Powder X-Ray Diffractometry and Scanning electron microscopy.A model of oxidized lowdensity lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus *** levels of nitric oxide,endothelial nitric oxide synthase,intracellular adhesion molecule-1,reactive oxygen species,superoxide dismutase,tumor necrosis factor-αand nuclear factor-κB were observed via assay ***:A total of 31 compounds were identified in ***-PC showed better anti-atherosclerotic power compared with PLF,moreover,enzyme linked immuneosorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis via antioxidant-related ***:Our findings elucidated that PLF-PC significantly enhanced the PLF’s efficacy on atherosclerosis.