Bemisia tabaci （Hemiptera： Aleyrodidae） nymphal feeding in cotton （Gossypium hirsutum） leaves

作     者：CHANG-CHI CHU DENNIS A. MARGOSAN JAMES S. BUCKNER THOMAS P. FREEMAN THOMAS J. HENNEBERRY 

作者机构：USDA ARS US Arid Land Agricultural Research Center Maricopa Arizona USDA ARS San Joaquin Valley Agricultural Science Center Parlier California Biosciences Research Laboratory Fargo North Dakota Electron Microscopy Center North Dakota State University Fargo North Dakota USA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2007年第14卷第5期

页      面：375-381页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

主　　题：Bemisia tabaci microscopy nymphs feeding stylet sheath stylet 

摘      要：We used brightfield electron microscopy （BEM）, differential interference contrast microscopy （DICM）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, and confocal laser scanning microscopy （CLSM） to investigate the stylet pathways of Bemisia tabaci during nymphal feeding behavior in cotton leaves beginning with penetration of the abaxial leaf surface and ending with stylets in sieve tubes in phloem tissues. Most nymphal stylets within salivary sheaths penetrating leaf tissues made complex turns and developed more than one salivary sheath branch before ending in sieve tubes. The external morphology of the salivary sheaths and their routes between and through leaf cells are described during the present study. Results showed the presence of the stylet within the sieve tubes. B. tabaci nymphs may remove stylets and feed in different sieve tubes. Ten short movies showing the progression of the stylet penetrations from adaxial surface to the sieve tubes are attached to Figures 8-15. The report and movies can be viewed from the internet. Download the movies to a local drive in your computer first for fast upload. The movies are posted on the website http：//***/Services/***？docid=14629. The movies can be used as a teaching aid in biology classes.