Correlation between slow transit constipation and spleen Qi deficiency, and gut microbiota: a pilot study

作     者：YU Zeyue HAO Liyu LI Zongyuan SUN Jianhui CHEN Hongying HUO Hairu LI Xiaoqin SHAN Zhongchao LI Hongmei YU Zeyue;HAO Liyu;LI Zongyuan;SUN Jianhui;CHEN Hongying;HUO Hairu;LI Xiaoqin;SHAN Zhongchao;LI Hongmei

作者机构：Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100007China Jiangsu UniversityZhenjiang 212013China Yunnan University of Traditional Chinese MedicineKunming 650504China Jiangxi University of Traditional Chinese MedicineNanning 330004China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第3期

页      面：353-363页

核心收录：

学科分类：100506[医学-中医内科学] 1006[医学-中西医结合] 1005[医学-中医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Fundamental Scientific Research Business Fees of Central Public Welfare Scientific Research Institutes of China Academy of Chinese Medical Sciences(No.Z2021022) National Natural Science(No.81891010):Study on the Authenticity of Traditional Chinese Medicine,Science and Technology Innovation Project of China Academy of Chinese Medical Sciences:Efficacy Evaluation and Mechanism Exploration of Huashi Baidu Granule based on"multi-link and multi-target"in the Prevention and Treatment of Influenza Virus Infection(CI2021A04605) Basic Scientific Research Business Fee Project of Central Public Welfare Scientific Research Institutes of the Chinese Academy of Chinese Medical Sciences:Study on the Novel Coronavirus Pneumonia Mechanism of Yiqi Zeng Fang and Huashi Baidu Granules(ZZ15-WT-04) 

主　　题：spleen Qi deficiency gastrointestinal microbiome probiotics fecal microbiota transplantation substance P vasoactive intestinal peptide slow transit constipation Maren Runchang pills 

摘      要：OBJECTIVE:To investigate the effect of slow transit constipation(STC)and spleen Qi deficiency on gut microbiota,and the mechanism underlying the action that the positive drug Maren Runchang(MR)alleviates ***:STC was induced,using the cathartic method of Senna and the hunger-fullness disorder method,in ICR mice;one group of model mice was treated with MR(6.24 g/kg).The changes in the general condition,fecal parameters,D-xylose content in the serum,intestinal propulsion rate,and histopathology of the colon were assessed after STC induction in the control,model,and MR *** microbiota transplantation(FMT)was performed from STC mice into pseudo germ-free *** in the contents of substance P(SP),vasoactive intestinal peptide(VIP),and gut microbiota in STC mice and pseudo germ-free mice were assessed after ***:Compared with the control group,the model mice showed the following results:the time of the first black stool was significantly longer(P0.01),the number and weight of black stools were significantly reduced within 6 h(P0.05),the D-xylose content in the serum was significantly reduced(P0.05),the intestinal propulsion rate decreased(P0.01),the content of VIP in colon tissue significantly increased(P0.05),and SP content in the colon tissue significantly decreased(P0.01);moreover,the colon showed significant inflamemation and ***,the abundance of Firmicutes was increased,the abundance of Bacteroides decreased,and the abundance of Alistipes,unclassified_o_Bacteroidales,Alloprevotella,Bilophila,and Anaerotruncus decreased,while the abundance of the conditional pathogenic bacteria Escherichia and Klebsiella ***,after treatment with MR,the time of the first black stool decreased(P0.01),the number of black stools within 6 h increased,and the intestinal propulsion rate increased(P0.05).Moreover,the content of D-xylose in the serum and the content of VIP in colon tissue significantly decreased(P0.05),the conte