Annals of Eye Science, 2018 7 © Annals of Eye Science. All rights reserved. Ann Eye Sci 2018;3:AB007 ***

作     者：Stéphanie Proulx 

作者机构：Centre de Recherche du CHU de Québec-UniversitéLavalAxe Médecine RégénératriceHôpital du Saint-SacrementQuébecCanada Centre de Recherche en Organogénèse Expérimentale de l'UniversitéLaval/LOEXQuébecCanada Département d'Ophtalmologie et d'Oto-Rhino-Laryngologie-Chirurgie Cervico-FacialeFacultéde MédecineUniversitéLavalQuébecCanada 

出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)

年 卷 期：2018年第1期

页      面：413-413页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：Tissue engineering choroid self-assembly 

摘      要：Background:The goal of this study was to engineer an epithelialized and endothelialized pigmented choroidal substitute using the self-assembly approach of tissue ***:Cells from human choroids were isolated and *** purity was assessed using immunostaining(CD31,HMB45,vimentin,keratins 8/18).To engineer the choroid,fibroblasts were cultured in the presence of serum and ascorbic acid to promote extracellular matrix(ECM)*** cells,melanocytes or RPE cells were separately seeded on the stromal *** substitutes were further characterized by histology,mass spectrometry,immunostaining,and compared to native human ***:The technique used to isolate choroidal cells yielded pure cultures of fibroblasts,melanocytes and vascular endothelial *** stromal substitutes engineered using the self-assembly approach were composed of collagen(types I,VI,XII and XIV),proteoglycans(decorin,lumican)and other ECM *** expression was confirmed using *** cells spontaneously assembled into capillary-like structures and vascular networks when cocultured with fibroblast-containing ECM ***:This study shows that the self-assembly approach of tissue engineering can be used to reconstruct a choroid using native *** model represents a unique tool to better understand the crosstalk between the different choroidal cell types and cell-ECM interactions.