AB014. What is the role of sildenafil in repairing retinopathy of prematurity?
作者机构：Department of PediatricsDivision of Newborn MedicineMontréal Children’s HospitalMcGill UniversityMontréalQCCanada Department of Ophthalmology and Neurology-NeurosurgeryMontréal Children’s Hospital-Research InstituteMcGill UniversityMontréalQCCanada
出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)
年 卷 期：2018年第1期
页 面：420-420页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Neonates hyperoxia retinopathy of prematurity(ROP) neuroscience vision health
摘 要：Background:Oxygen therapy provided to support the lungs of premature newborns often leads to damages to the retina called retinopathy of prematurity(ROP)and long-term visual *** treatments for ROP are invasive and aim at preventing further progression of the damages to the retina,but do not repair these *** goal is to investigate the therapeutic effect of sildenafil on retinal structure in a rat model of ***:Sprague-Dawley rats were exposed to hyperoxia(i.e.,80%oxygen)interrupted by three 0.5-hour periods of normoxia(i.e.,21%oxygen)(hyperoxic animals)per day or room air only(i.e.,21%oxygen)(control animals)from post-natal day 4(P4)to 14(P14).Pups were then housed in room ***(50 mg/kg)or vehicle was given per os twice daily after oxygen exposure(from P15 to P21).At P30,retinas were extracted,and *** retinal histology,eyes were stained with toluidine blue to measure the thicknesses of the different retinal *** was also performed to count the number of retinal ganglion cells and bipolar cells in the inner retina,as well as the number of astrocytes and microglia within the different retinal ***:Hyperoxia caused a reduction in thickness of the outer plexiform layer(OPL)and a decrease in the number of bipolar cells in some parts of the retina,compared to control animals(P0.05);in addition,the number of microglia cells was significantly increased in the rats exposed to hyperoxia,compared to controls(P0.05).Sildenafil improved OPL thickness in ROP animals,but did not change the number of bipolar *** hyperoxic rats treated with sildenafil,the number of microglia were similar to control *** number of retinal ganglion cells and astrocytes did not differ significantly between the ***:Treatment with sildenafil following oxygen exposure provided some recovery of the structure of the *** beneficial effect may be modulated by a decrease of inflammation within t
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