*** of a novel anti-angiogenic protein for the treatment of exudative age-related macular degeneration

作     者：Erika Hooker Natalija Popovic Andrea Barabino Anthony Flamier Gilbert Bernier Bruno Larrivée 

作者机构：Department of BiochemistryMaisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada Department of OphthalmologyMaisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada 

出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)

年 卷 期：2018年第1期

页      面：429-429页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Age-related macular degeneration(AMD) angiogenesis 

摘      要：Background:Age-related macular degeneration(AMD)is a leading cause of blindness in *** exudative(wet)form of AMD accounts for approximately 15%of AMD patients,but is responsible for the majority of severe vision loss associated with the *** AMD is characterized by choroidal neovascularization,the abnormal growth of blood vessels from the choroid into the sub-retinal *** therapies for exudative AMD directly target and inhibit the vascular endothelial growth factor(VEGF)signaling pathway,a signaling axis that promotes endothelial cell *** initially effective at restoring visual acuity,recent studies suggest that chronic use of anti-VEGF therapies can lead to further vision impairment through off-target effects on photoreceptors and other non-vascular *** off-target effects of anti-VEGF therapy highlights the need for alternative treatments for this increasingly common *** have recently identified a novel anti-angiogenic protein,AAP1 that inhibits retinal angiogenesis during *** a regulator of the vasculature,our current work aims to characterize the function of AAP1 in endothelial cells and determine the potential of AAP1 as a therapy for exudative ***:To address our aims,we used various in vivo and in vitro models of normal and pathological vascular *** were injected intravitreally with AAP1 to investigate its effects on developmental and pathological *** HUVECs,we employed immunofluorescent quantifications to determine the impact of AAP1 on sprouting angiogenesis by measuring cellular proliferation,apoptosis and *** investigate the signaling events that mediate the actions of AAP1,we examined key signaling pathways involved in angiogenesis by western blotting and ***:We evaluated the role of AAP1 as a regulator of angiogenesis during retinal development and mouse models of *** pilot data show that AAP1 prevents angiogenesis in vitro and in vivo,and th