Antibacterial and anti-parasitic activities of Terfezia claveryi methanolic extract against some common pathogenic agents of infectious diarrhea

作     者：Sultan F Alnomasy Sultan F Alnomasy

作者机构：Department of Medical Laboratories SciencesCollege of Applied Medical SciencesShaqra UniversityAl-Quwayiyah 19257Saudi Arabia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2022年第12卷第5期

页      面：216-222页

核心收录：

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Terfezia claveryi Shigellosis Giardiasis Herbal medicines Infectious diarrhea Cellular mechanisms Cytotoxicity Microbiology 

摘      要：Objective:To assess the antidiarrheal effects of Terfezia claveryi methanolic extract against Escherichia coli,Salmonella typhimurium,Shigella flexneri,and Giardia ***:Antibacterial effects of the Terfezia claveryi methanolic extract were carried out by determining the minimum inhibitory concentration(MIC)and minimum bactericidal concentration through micro broth dilution ***,reactive oxygen species production and protein leakage were *** evaluate the in vitro anti-giardial effects of Terfezia claveryi methanolic extract,Giardia lamblia WB(ATCC®30957)trophozoites were treated with various concentrations of Terfezia claveryi methanolic extract for 10-360 *** addition,the plasma membrane permeability of trophozoites treated with Terfezia claveryi methanolic extract was *** cytotoxicity effects of Terfezia claveryi methanolic extract against normal(HEK293T)and cancer(MCF-7)cells were also assessed using the MTT ***:The MIC and minimum bactericidal concentration of Terfezia claveryi methanolic extract against bacterial strains were in the range of 0.52-1.04 and 1.04-2.08 mg/mL,*** results revealed that reactive oxygen species production and protein leakage were significantly increased after the bacteria were treated with the Terfezia claveryi methanolic extract,especially at 1/3 and 1/2 MICs(P0.001).Furthermore,Terfezia claveryi methanolic extract decreased the viability of Giardia lamblia trophozoites in a dose-dependent *** claveryi methanolic extract at 1,2,and 4 mg/mL resulted in 100%mortality in Giardia lamblia trophozoites after 360,240,and 120 min,***,Terfezia claveryi methanolic extract altered the permeability of plasma membrane of Giardia lamblia trophozoites by increasing the *** assay revealed that the 50%cytotoxic concentrations values for HEK293T and MCF-7 cells were 4.32 mg/mL and 6.40 mg/mL,respectively,indicating that Terfezia clavery