Single-cell RNA sequencing reveals the landscape of maize root tips and assists in identification of cell type-specific nitrate-response genes

作     者：Xuhui Li Xiangbo Zhang Shuai Gao Fangqing Cui Weiwei Chen Lina Fan Yongwen Qi Xuhui Li;Xiangbo Zhang;Shuai Gao;Fangqing Cui;Weiwei Chen;Lina Fan;Yongwen Qi

作者机构：Institute of Nanfan&Seed IndustryGuangdong Academy of ScienceGuangzhou 510316GuangdongChina College of Agriculture and BiologyZhongkai University of Agriculture and EngineeringGuangzhou 510325GuangdongChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第6期

页      面：1589-1600页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：financially supported by the Special Project of Guangdong Academy of Sciences,China(2020GDASYL-20200103073) the Special Project for Research and Development in Key areas of Guangdong Province(2019B020238001) the National Natural Science Foundation of China(32072027) 

主　　题：Maize Single-cell RNA sequencing Root Nitrate 

摘      要：The root system is fundamental for maize growth and *** its heterogeneity and cell type-specific response to nitrate at the single-cell level will shed light on root development and nutrient *** profiled the transcriptomes of7000 cells derived from root tips of maize seedlings grown on media with or without nitrate,and identified 11 major cell types or tissues and 85 cell type-specific nitrate-response genes,including several known nitrate metabolic genes.A pseudotime analysis showed a continuous pseudotime series with the beginning at meristematic zone cells and showed that the root hair cell was derived by differentiation of a subset of epidermal *** comparison of root cells between maize and rice revealed the conservation and divergence of the root cell types and identified 57,216,and 80 conserved orthologous genes in root hair,endodermis,and phloem cells *** study provides a global view of maize root tip developmental processes and responses to nitrate at the single-cell *** genes described in the present work could serve as targets for further genetic analyses and accurate regulation of gene expression and phenotypic variation in specific cell types or tissues.