Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents

作     者：Zhaonan Li Chuan Tian Dechao Jiao Jing Li Yahua Li Xueliang Zhou Huiping Zhao Yanan Zhao Xinwei Han 

作者机构：Department of Interventional RadiologyFirst Affiliated Hospital of Zhengzhou UniversityZhengzhou450001PR China Department of RadiologyFirst Affiliated Hospital of Zhengzhou UniversityZhengzhou450001PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第3期

页      面：266-280页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

基　　金：This work was supported by the Young and middle-aged health science and technology innovation talent project of Henan Province(YXKC2020037) the Henan Medical Science and Technology Public Relations Program(SB201902014)and the Horizontal Research Program of Zhengzhou University(Grant No.24110005). 

主　　题：Anti-inflammatory Antihyperplasia Airway stent Silver nanoparticle Cisplatin 

摘      要：Anti-inflammatory and antihyperplasia activities are essential requirements for the successful use of airway stents.In this work,silver nanoparticles(AgNPs)and cisplatin(DDP)were combined in a synergistic modification strategy to improve the surface function of airway stents.Using polycaprolactone(PCL)as a drug carrier,a dual-functional PCL-AgNPs-DDP fiber film-coated airway stent was fabricated by electrospinning.The physicochemical and biological properties of the obtained fiber films were examined.The ATR-FTIR,XPS,SEM-EDS and TEM results suggested that AgNPs and DDP could be successfully immobilized onto the airway stent surface.The drug release and surface degradation results revealed that AgNPs and DDP can undergo sustained release from films for 30 d,and the weight loss was approximately 50%after 35 d.In addition,the dual-functional fiber film suppressed human embryonic lung fibroblast growth and exhibited excellent antibacterial activity against Staphylococcus aureus,Pseudomonas aeruginosa and Candida albicans.Furthermore,the effectiveness of the dual-functional fiber film-coated airway stent was evaluated by application to the trachea of New Zealand rabbits.The in vivo results indicated that PCL-AgNPs-DDP fiber film-coated airway stent can significantly inhibit granulation tissue formation and collagen deposition,reduced the expression of IL-8,TNF-α,IL-1α,PCNA,α-SMA and CD68,and ultimately achieved anti-inflammatory and antihyperplasia effects.Hence,this study provides a dual-functional surface-coated airway stent to address the clinical complications associated with respiratory tract inflammation and granulation tissue hyperplasia,thus inhibiting tracheal stenosis.