Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells
作者机构：Laboratory of Stem Cell and Tissue EngineeringOrthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of Cardiovascular SurgeryWest China HospitalSichuan UniversityChengduSichuan610041China Department of Cardiovascular SurgeryShandong Provincial HospitalShandong First Medical UniversityJinanShandong250021China Research Core FacilityWest China HospitalSichuan UniversityChengduSichuan610041China
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2022年第7卷第8期
页 面：206-218页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学]
基 金：supported by the National Key R&D Program of China(Grant No.2017YFC1104702) National Natural Science Foundation of China(Grant No.31771065) the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002).
主 题：Congenital heart disease Polypyrrole Polyurethane/small intestinal submucosa Hypoxia Urine-derived stem cells
摘 要：Approximately 25%of patients with congenital heart disease require implantation of patches to repair.However,most of the currently available patches are made of inert materials with unmatched electrical conductivity and mechanical properties,which may lead to an increased risk for arrhythmia and heart failure.In this study,we have developed a novel Polyurethane/Small intestinal submucosa patch(PSP)with mechanical and electrical properties similar to those of the native myocardial tissue,and assessed its feasibility for the reconstruction of right ventricular outflow tract.A right ventricular outflow tract reconstruction model was constructed in 40 rabbits.Compared with commercially available bovine pericardium patch,the PSP patch has shown better histocompatibility and biodegradability,in addition with significantly improved cardiac function.To tackle the significant fibrosis and relatively poor vascularization during tissue remodeling,we have further developed a bioactive patch by incorporating the PSP composites with urine-derived stem cells(USCs)which were pretreated with hypoxia.The results showed that the hypoxia-pretreated bioactive patch could significantly inhibit fibrosis and promote vascularization and muscularization,resulting in better right heart function.Our findings suggested that the PSP patch combined with hypoxia-pretreated USCs may provide a better strategy for the treatment of congenital heart disease.
维普期刊数据库