Determining the protective effects of Ma-Mu-Ran Antidiarrheal Capsules against acute DSS-induced enteritis using 16S rRNA gene sequencing and fecal metabolomics

作     者：ZHENG Si-Li ZHANG Dong-Ning DU AN Yan-Fen HUANG Fang HAN Lin-Tao MO Guo-Yan ZHENG Si-Li;ZHANG Dong-Ning;DUAN Yan-Fen;HUANG Fang;HAN Lin-Tao;MO Guo-Yan

作者机构：Pharmacy FacultyHubei University of Chinese MedicineWuhan 430065China College of Basic Medical SciencesHubei University of Chinese MedicineWuhan 430065China Xinjiang Uygur Pharmaceutical Co.Ltd.UrumqiXinjiang 830026China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2022年第20卷第5期

页      面：364-377页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Tianchi plan of introducing high-level talents in the Xinjiang Uygur Autonomous Region(2019-39) Major projects of Xinjiang Uygur Autonomous Region“Research and development of new ethnic drugs and large varieties”(2016A03005-2) 

主　　题：Acute enteritis Ma-Mu-Ran Antidiarrheal Capsules 16S rRNA sequencing Fecal metabolomics Gut microbiota 

摘      要：Ma-Mu-Ran Antidiarrheal Capsules(MMRAC)is traditional Chinese medicine that has been used to treat diarrhea caused by acute enteritis(AE)and bacillary dysentery in Xinjiang(China)for many ***,the potential therapeutic mechanism of MMRAC for AE and its regulatory mechanism on host metabolism is *** study used fecal metabolomics profiling with GC/MS and 16S rRNA gene sequencing analysis to explore the potential regulatory mechanisms of MMRAC on a dextran sulfate sodium salt(DSS)-induced mouse model of *** metabolomics-based analyses were performed to detect the differentially expressed metabolites and metabolic *** 16S rRNA gene sequencing analysis was used to assess the altered gut microbes at the genus level and for functional ***,Pearson correlation analysis was used to integrate differentially expressed metabolites and altered bacterial *** results revealed that six intestinal bacteria and seven metabolites mediated metabolic disorders(i.e.,metabolism of amino acid,carbohydrate,cofactors and vitamins,and lipid)in AE ***,ten altered microbes mediated the differential expression of eight metabolites and regulated these metabolisms after MMRAC ***,these findings demonstrate that AE is associated with metabolic disorders and microbial ***,we present that MMRAC exerts protective effects against AE by improving host metabolism through the intestinal flora.