Bis-cyclometalated Ir(Ⅲ)Complex-Based Electrogenerated Chemiluminescence Sensor Array for Discriminating Three Biothiols
作者机构：Key Laboratory of Analytical Chemistry for Life Science of Shaanxi ProvinceSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’an 710062People’s Republic of China
出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)
年 卷 期：2020年第4卷第2期
页 面：114-121页
核心收录：
学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学]
基 金：the National Natural Science Foundation of China(Nos.21775097,21775098) the Fundamental Research Funds for the Central Universities(GK201801006)。
主 题：ECL Sensor array Biothiols Ir(Ⅲ)complex Principal component analysis
摘 要：A simple and sensitive electrogenerated chemiluminescence(ECL)sensor array was developed for the discrimination of three biothiols,including homocysteine(Hcy),cysteine(Cys)and glutathione(GSH)using two bis-cyclometalated Ir(Ⅲ)complexes as ECL probes.Two aldehyde groups bearing bis-cyclometalated Ir(Ⅲ)complexes were selected as ECL probes,including[(bt)2Ir(phen-CHO)]PF_(6)(bt=2-phenylbenzothiazole,phen-CHO=1,10-phenanthroline-5-carboxaldehyde,probe 1)and[(ppy)_(2)Ir(phen-CHO)]PF_(6)(ppy=2-phenylpyridine,probe 2).A“signal onECL method was proposed for biothiols assay based on the increase in ECL intensity of two ECL probes by biothiols.Three biothiols can be detected with detection limits of 0.8,0.7 and 0.8μM by probe 1 and 0.3,0.4 and 0.5μM by probe 2 for Cys,Hcy and GSH,respectively.Two-element sensor array was assembled for the detection of three biothiols due to the different enhancement effects of biothiols on the ECL intensity of two ECL probes.The ECL sensor array combining with the principal component analysis was applied to discriminate three biothiols.This study demonstrates that the ECL sensor array using dual ECL reagents provides a promis-ing way for the discrimination of multiple biothiols with good sensitivity and simpleness.
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