On the use of residual dipolar couplings in multi-state structure calculation of two-domain proteins

作     者：Alexandra Born Morkos A.Henen Parker J.Nichols Beat Vogeli Alexandra Born;Morkos A.Henen;Parker J.Nichols;Beat V?geli

作者机构：University of Colorado Anschutz Medical CampusDepartment of Biochemistry and Molecular Genetics12801 East 17th AvenueAuroraCO80045USA Faculty of PharmacyMansoura UniversityMansoura35516Egypt 

出 版 物：《Magnetic Resonance Letters》 (磁共振快报（英文）)

年 卷 期：2022年第2卷第2期

页      面：61-68,I0002页

核心收录：

学科分类：07[理学] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学] 

基　　金：supported by NIH Grant R01GM130694-01A1,a start-up package by the University of Colorado to B.V.,University of Colorado Cancer Center Support Grant P30 CA046934 NIH Biomedical Research Support Shared Grant S10 OD025020-01 

主　　题：RDC Residual dipolar coupling Two-domain protein Multi-state structure 

摘      要：Residual dipolar couplings(RDCs)are powerful nuclear magnetic resonance(NMR)probes for the structure calculation of ***,an alignment tensor that defines the orientation of the entire molecule relative to the magnetic field is determined either before refinement of individual bond vectors or simultaneously with this *** single-domain proteins this approach works well since all bond vectors can be described within the same coordinate frame,which is given by the alignment ***,novel approaches are sought after for systems where no universal alignment tensor can be ***,we present an approach that can be applied to two-domain proteins that enables the calculation of multiple states within each domain as well as with respect to the relative positions of the two domains.