Extracts of Portulaca oleracea promote wound healing by enhancing angiology regeneration and inhibiting iron accumulation in mice

作     者：Jinglin Guo Juan Peng Jing Han Ke Wang Ruijuan Si Hui Shan Xiaoying Wang Ju Zhang Jinglin Guo;Juan Peng;Jing Han;Ke Wang;Ruijuan Si;Hui Shan;Xiaoying Wang;Ju Zhang

作者机构：School of NursingQingdao UniversityQingdao 266071China Surgery of Traditional Chinese MedicineSecond Affiliated Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300150China School of PharmacyQingdao UniversityQingdao 266071China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2022年第14卷第2期

页      面：263-272页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Youth Program of the National Natural Science Foundation of China[grant No.8170 1838] 

主　　题：deep tissue pressure injur ischemia-reperfusion Portulaca oleracea L. tissue repair wound healing 

摘      要：Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid *** number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in *** iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron ***,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively ***:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory *** results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.