Extracts of Portulaca oleracea promote wound healing by enhancing angiology regeneration and inhibiting iron accumulation in mice
Extracts of Portulaca oleracea promote wound healing by enhancing angiology regeneration and inhibiting iron accumulation in mice作者机构:School of NursingQingdao UniversityQingdao 266071China Surgery of Traditional Chinese MedicineSecond Affiliated Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300150China School of PharmacyQingdao UniversityQingdao 266071China
出 版 物:《Chinese Herbal Medicines》 (中草药(英文版))
年 卷 期:2022年第14卷第2期
页 面:263-272页
学科分类:1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金:supported by the Youth Program of the National Natural Science Foundation of China[grant No.8170 1838]
主 题:deep tissue pressure injur ischemia-reperfusion Portulaca oleracea L. tissue repair wound healing
摘 要:Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid *** number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in *** iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron ***,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively ***:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory *** results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.