Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

作     者：Xiaoyu Xue Muya Shu Zhifeng Xiao Yannan Zhao Xing Li Haipeng Zhang Yongheng Fan Xianming Wu Bing Chen Bai Xu Yaming Yang Weiyuan Liu Sumei Liu Jianwu Dai Xiaoyu Xue;Muya Shu;Zhifeng Xiao;Yannan Zhao;Xing Li;Haipeng Zhang;Yongheng Fan;Xianming Wu;Bing Chen;Bai Xu;Yamning Yang;Weiyuan Liu;Sumei Liu;Jianwu Dai

作者机构：State Key Laboratory of Molecular Developmental BiologyInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100190China University of Chinese Academy of SciencesBeijing100190China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第4期

页      面：757-769页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China (81891000) the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16040700) the National Key R&D Program of China (2017YFA0104701, 2017YFA0104704 and 2016YFC1101501) the Jiangsu Key R&D Program (BE2018664) 

主　　题：Nestin ependymal cells spinal cord injury astrocytes pericytes 

摘      要：Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury(SCI), Nestin expression is reactivated and Nestinpositive(Nestin;) cells aggregate at the injury site. However, the derivation of Nestin;cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamoxifen inducible CreER(T2)-loxP system, we verified that ependymal cells contribute few Nestin;cells either to the lesion core or the lesion edge after SCI. In the lesion edge, GFAP+astrocytes were the main cell type that expressed Nestin;they then formed an astrocyte *** the lesion core, Nestin;cells expressed αSMA or Desmin, indicating that they might be derived from pericytes. Our results reveal that Nestin;cells in the lesion core and edge came from various cell types and rarely from ependymal cells after complete transected SCI, which may provide new insights into SCI repair.