Gama-aminobutyric acid(GABA)alleviates hepatic inflammation via GABA receptors/TLR4/NF-kB pathways in growing-finishing pigs generated by super-multiparous sows

作     者：Shumin Zhang Jinbiao Zhao Jinhua Hu Hengxun He Yihan Wei Linbao Ji Xi Ma Shumin Zhang;Jinbiao Zhao;Jinhua Hu;Hengxun He;Yihan Wei;Linbao Ji;Xi Ma

作者机构：State Key Laboratory of Animal NutritionCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第9卷第2期

页      面：280-290页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：the National Natural Science Foundation of China(31930106,31829004 and 31722054) the 2115 Talent Development Program of China Agricultural University(1041-00109019) the China National Postdoctoral Program for Innovation Talents(BX20200365) China Postdoctoral Science Foundation(2020M680771) the National Ten-thousand Talents Program of China(23070201) the project of Academician Workstation in Chengdejiuyun Agricultural and livestock Co.,Ltd(199A7310H) 

主　　题：Gama-aminobutyric acid Growing-finishing pig Liver inflammation Lipopolysaccharide NF-kB 

摘      要：The offspring of super-multiparous sows face problems such as decreased growth performance,poor meat quality and even diseases in animal ***-aminobutyric acid(GABA)has long been known to promote growth and suppress inflammation,but little is known about the mechanisms.A total of 72 growing-finishing pigs from the 8th generation were randomly allotted to 2 groups with 6 replicates per treatment to receive a cornesoybean basal diet or the basal diet supplemented 20 mg/kg GABA for 60 *** the animal-trial period,samples of serum and liver were collected for further ***,a lipopolysaccharide(LPS)-induced inflammatory model using HepG2 cells was established to explore the role of GABA on regulating hepatic *** results indicated that inflammatory cell infiltration occurs in the liver of progeny of super-multiparous sows,and dietary supplementation with GABA influenced liver morphology,increased activities of antioxidant enzymes and decreased the expression abundance of pro-inflammatory cytokines,including tumor necrosis factor-α(TNFα)and interleukin(IL)-1β,in the liver of growing-finishing pigs(P0.05).In addition,GABA supplementation increased mRNA expressions of peroxisome proliferator-activated receptor g(PPARg)and GABA receptors(GABARs),and reduced the expression of toll-like receptor 4(TLR4)/nuclear factor-kB(NF-kB)signaling(P0.05).Additionally,an in vitro experiment demonstrated that GABA decreased the expressions of hepatic TLR4/NF-kB signaling via activating GABARs under LPS-stress(P0.05).In summary,liver injury may affect the growth performance of growing-finishing pigs by changing hepatic mitochondrial metabolism,the expression of pro-inflammatory cytokines and TLR4/NF-kB pathway and that GABA supplementation has a restorative effect by acting on GABARs.