Heterotrimeric G protein participated in modulation of cytoplasmic calcium concentration in pollen cells

作     者：SHANG Zhonglin, MA Ligeng, WANG Xuechen and SUN Daye (1. The College of Biological Sciences, Hebei Normal University, Shijiazhuang 050016, China 2. State Key Laboratory of Plant Physiology & Biochemistry, Beijing 100094, China) 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2003年第9期

页      面：40-44+84页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No.39930230) the Major State Basic Research Development Program of China (G1999011700) and the National Fund for Distinguished Young Scholars (No.30025024) 

主　　题：heterotrimeric G protein Lilium daviddi pollen cytoplasmic calcium. 

摘      要：Cytoplasmic free calcium concentration([Ca2+]c) in pollen cells of Lilium daviddi is measured with confocal laserscanning microscopy to investigate the effect of heterotrimeric G protein （G protein） on [Ca2+]cand the possible signal transduction path-way of G protein triggering cellular calcium signal. After application, cholera toxin （CTX）, an agonist of G protein, triggers a transientincrease of [Ca2+]cin pollen cells, and evokes a spatial-temporal characteristic calcium dynamics; while pertussis toxin （PTX）, a G pro-tein antagonist, leads to the decrease of [Ca2+]c. Both L-type Ca2+channel blocker verapamil and inhibitor of IP3receptor heparin inhibitCTX-induced [Ca2+]cincrease. The results show that G protein may play a role in the modulation of [Ca2+]cthrough enhancing the ex-tracellular Ca2+influx and releasing of Ca2+from intracellular stores.