隔离环境下通过胚胎移植获得无菌状态的小鼠
Germfree status of mice obtained by embryo transfer in anisolator environment出 版 物：《中国比较医学杂志》 (Chinese Journal of Comparative Medicine)
年 卷 期：2006年第16卷第7期
页 面：423-423页
学科分类：10[医学]
主 题：MICE GERMFREE EMBRYOTRANSFER ISOLATOR
摘 要：The technique of embryo transfer has been evaluated for the purpose of changing the mouse stocks to a germfree(GF)*** results showreproducible and quality-assured conversion of animalstothose which are negativeforthe presence of *** and easy access to GF mice is advantageous for studies of selected microflora andtheir cross-talks withthe host,when applying,***,proteomic and metabolic *** studyinvolved embryotransfer in anisolator environment,thereby allowingimplantationof cleansed embryosinto GFrecipients under well-controlled *** recipientfemales gave birth normally andtookcare of the offspringasif they were their own pups,thus enhancing the survival *** to full technical resources required to maintain GFisolators are,however,a *** this study,we used stainless steel isolators designed by Gustafsson(1959),on which a stereomicroscope wasmountedtofacilitate embryo transfer inside the *** use of embryo transfer and isolator techniques will facilitate the availability ofvarious mouse mutant models under different gnotobiotic conditions,GF,monoxenic or polyxenic animals,to enable comparison withconventional animals for physiological and pathophysiological studies.
维普期刊数据库
同方期刊数据库