Transforming Activity of a Novel Mutant of HPV16 E6E7 Fusion Gene

作     者：Qiang Xie Zhi-xiang Zhou Ze-lin Li Yi Zeng 

作者机构：（College of Life Science and Bioengineering Beijing University of Technology Beijing 100124 China） 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2011年第26卷第3期

页      面：206-213页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：National 863 Program(2007AA021107) Beijing Municipal Commission of Education Research Projects(KM201010005008) Doctoral Start-up Foundation of Beijing University of Technology 

主　　题：Human papillomavirus (HPV) E6 E7 Gene optimization Gene mutation Transformation 

摘      要：An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost the transformation capability to NIH 3T3 cells and tumorigenicity in SCID mice, but also maintained very good stability and antigenicity. These results suggests that the HPV16 omfE6E7 gene should undergo further study for application as a safe antigen-specific therapeutic vaccine for HPV16-associated tumors.