REAL-TIME MONITORING OF MITOCHONDRIAL FUNCTION AND CEREBRAL BLOOD FLOW FOLLOWING FOCAL ISCHEMIA IN RATS

作     者：AMIR LIVNAT MICHAEL TOLMASOV EFRAT BARBIRO-MICHAELY AVRAHAM MAYEVSKY 

作者机构：The Mina&Everard Goodman Faculty of Life-Sciences and The Gonda Multidisciplinary Brain Research Center Bar-Ilan University Ramat-Gan52900Israel 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2008年第1卷第1期

页      面：63-69页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 10[医学] 0702[理学-物理学] 

主　　题：Mitochondrial function cerebral blood flow focal ischemia 

摘      要：Focal ischemia due to reduction of cerebral blood flow(CBF),creates 2 zones of damage:the core area,which suffers severe damage,and penumbra area,which surrounds the core and suffers intermediate levels of injury.Objectives:A novel method is introduced,which evaluates mitochondrial function in the core and in the penumbra,during focal cerebral ischemia.Methods:Wistar rats underwent focal cerebral ischemia by middle cerebral artery occlusion(MCAO)for 60 minutes,followed by 60 minutes of reperfusion.Mitochondrial function was assessed by a unique Multi-Site—Multi-Parametric(MSMP)monitoring system,which measures mitochondrial NADH using fluorometric technique,and CBF using Laser Doppler Flowmetry(LDF).Results:At the onset of occlusion,CBF dropped and NADH increased significantly only in the right hemisphere.CBF levels were significantly lower and NADH significantly higher in the core than in the penumbra.After reperfusion,CBF and NADH recovered correspondingly to the intensity of ischemia.Conclusion:Application of the MSMP system can add significant information for the understanding of the cerebral metabolic state under ischemic conditions,with an emphasis on mitochondrial function.