Modulation of presynaptic nAChRs on postsynaptic GABA receptor in optic tectum of juvenile Xenopus

作     者：WANG Hong & CAI Haoran (TSAI Haojan) First Hospital, Peking University, Beijing 100034, China Correspondence should be adressed to Cai Haoran (e-mail: hjtsai@ ***) 

作者机构：First Hospital Peking University China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2003年第48卷第5期

页      面：417-423页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：the Na-tional Natural Science Foundation of China (Grant No.39670774) 

主　　题：视觉分析 神经原突触前 突触后 伽马氨基丁酸 感受器 神经调节 青少年 

摘      要：Using the blind patch-clamp technique with the whole-cell mode, we have studied the modulation of pre-synaptic receptor on postsynaptic g-aminobutyric acid (GABA) receptor measuring miniature inhibitory postsy-naptic currents (mIPSCs) in optic tectum of Xenopus during critical peroid. It was demonstrated that compared with mature neurons, mIPSCs recorded from immature neurons had smaller amplitude and longer decay time. mIPSCs are mediated by GABAa receptor. The nicotinic acetylcholine receptor agonists (carbachol, cytisine, nicotine, DMPP and so on) could increase the frequency of mIPSCs. The enhance-ment of mIPSCs frequency induced by nAChR agonists was calcium-dependent. However, the choline, a product of hy-drolyzed acetylcholine, could not increase the frequency of mIPSCs. DH-b-E, a competitive antagonist of nAChR, blocked the increase of mIPSCs frequency induced by car-bachol. Mecamyllamine, an a3b4 subtype of nAChR antago-nist, also blocked the carbachol-induced enhancement of mIPSCs. On the other hand, MLA, a7 subtype of nAChR antagonist, had no effect on it. Thus, it seems that nAChR could presynaptically modulate the mIPSCs and a3b4 sub-type of nAChR might be involved. But a7 nAChR subtype of nAChR would not be involved. The modulation is calcium- dependent. Meanwhile, we found that Ca2+-free solution could elicit giant PSCs. The frequency of mIPSCs also is related with the level of HP.