Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time–tandem mass spectrometry
Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time–tandem mass spectrometry作者机构:Medical Experiment and Analysis Center of PLA General Hospital ASPEC Technologies Limited Beijing
出 版 物:《Acta Pharmaceutica Sinica B》 (药学学报(英文版))
年 卷 期:2015年第5卷第5期
页 面:482-486页
核心收录:
学科分类:1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100706[医学-药理学] 1001[医学-基础医学(可授医学、理学学位)] 100602[医学-中西医结合临床] 10[医学]
主 题:3-Nitrotyrosine 3-Chlorotyrosine Determintion DART–MS/MS Human plasma
摘 要:A novel method for the simultaneous determination of 3-nitrotyrosine(NT) and 3-chlorotyrosine(CT) in human plasma has been developed based on direct analysis in real time–tandem mass spectrometry(DART–MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring(MRM) of the ion transitions at m/z 216.2/170.1 for CT, m/z 227.2/181.1 for NT and m/z 230.2/184.2 for the internal standard, d^3-NT. The assay was linear in the ranges 0.5–100 μg/m L for CT and 4–100 μg/m L for NT with corresponding limits of detection of 0.2 and 2 μg/m L. Intra- and inter-day precisions and accuracies were respectively 15% and ±15%. Matrix effects were also evaluated. The method is potentially useful for high throughput analysis although sensitivity needs to be improved before it can be applied in clinical research.