Human embryonic stem cell lines derived from the Chinese population

作     者：Zhen Fu FANG1,2, Fan JIN3, Hui GAI1, Ying CHEN1, Li WU1, Ai Lian LIU1, Bin CHEN1, Hui Zhen SHENG1,2,* 1Center for Developmental Biology, Xinhua Hospital, Shanghai Second Medical University, 1665 Kong Jiang Road, Shang- hai 200092, China 2Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 3In vitro Fertilization Center of Women’s Hospital, School of Medicine, Zhejiang University, Zhejiang 310006, China 

作者机构：CenterforDevelopmentalBiologyXinhuaHospitalShanghaiSecondMedicalUniversity1665KongJiangRoadShanghai200092 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2005年第15卷第5期

页      面：394-400页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：grantsfromNationalBasicResearchProgramofChina(973Program)(No:001CB509903,001CB509904) Hi-TechResearchandDevelopmentProgramofChina(973Program)(No:2001AA216121,2004AA205010) NationalNaturalScienceFoundationofChina(No:30040003) S 

主　　题：human embryonic stem cell pluoripotent cell line teratoma. 

摘      要：Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.