Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress

作     者：Xiao-liang LI Yue KANG Xiao-yan ZHANG Bing-lin ZHU Wei-huan FANG 

作者机构：Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary MedicineCollege of Animal SciencesZhejiang UniversityHangzhou 310058China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2012年第13卷第6期

页      面：465-477页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Project (No.20082332Y03) supported by the Science and Technology Department of Hangzhou City China 

主　　题：Pelodiscus sinensis Heat shock cognate protein 70 (Hsc70) Phylogenetic analysis Heat stress 

摘      要：The heat shock cognate protein 70 (Hsc70) is a member of a 70-kDa heat shock protein (HSP70) family that functions as molecular *** this study,a novel Hsc70 gene from Chinese soft-shelled turtle (Pelodiscus sinensis) (tHsc70) was *** tHsc70 full-length complementary DNA (cDNA) is 2 272 bp long with a 1 941-bp open reading frame (ORF) encoding 646 amino *** characteristic signature regions of the HSP70 family,two major domains of an adenosine triphosphate (ATP)/guanosine triphosphate (GTP) binding domain (ABD),and a substrate-binding domain (SBD) were present in the predicted tHsc70 amino acid *** tHsc70 gene was expressed in Escherichia coli BL21 and the expression product reacted with the anti-Hsc70 mouse monoclonal antibody by Western *** analysis revealed that tHsc70 shared identity from 53.9% to 87.7% at the nucleotide level,and 49.1% to 99.5% at the amino acid level with the known *** analysis showed that tHsc70 was clustered together with the Hsc70 gene of another reptile species (Alligator mississippiensis).The tHsc70 was expressed in the liver,lung,heart,and skeletal *** expression patterns of tHsc70 messenger RNA (mRNA) differed among different tissues under different durations of heat stress at 40 °*** at 25 °C for 1 h after heat stress was also different among tissues and length of heat *** of different profiles of expression under heat stress,tHsc70 may play roles in protecting turtles from thermal stress.