The recruitment of exogenous endothelial progenitor cells in lung tumor model of nude mice

作     者：Qiang Peng Ming Liu Shu-Min Song Xian-Hua Li Yi-Hua Du Yong Zhi Min-Yong Wang 

作者机构：Department of Surgery Affiliated Hospital of Luzhou Medical College Luzhou Sichuan 646000 P. R. China Physiology Laboratory Luzhou Medical College Luzhou Sichuan 646000 P. R. China Central Laboratory Luzhou Medical College Luzhou Sichuan 646000 P. R. China 

出 版 物：《Chinese Journal of Cancer》 (CHINESE JOURNAL OF CANCER)

年 卷 期：2010年第29卷第11期

页      面：952-958页

核心收录：

学科分类：0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 071003[理学-生理学] 0713[理学-生态学] 

基　　金：Chinese National Natural Science Foundation Program (No.30700876) SED project (No.2006B026) 

主　　题：内皮祖细胞 肺肿瘤 外源性 血管内皮生长因子受体 缺氧诱导因子-1α 裸鼠 招聘 模型 

摘      要：Background and Objective: Endothelial progenitor cells (EPCs) play an important role in hypoxia-triggered tumor vasculogenesis. However, the homing of exogenous EPCs in tumors is still unclear. In this study, we investigated the recruitment of exogenous EPCs in human lung adenocarcinoma model of nude mice. Methods: EPCs labeled with green fluorescence protein (GFP) were transplanted into nude mice bearing human lung adenocarcinoma. The growth of tumor was observed. After the mice were killed, GFP-EPCs in different tissues were examined by fluorescence. The tumor tissues were stained for CD133, hypoxia-inducible factor-1alpha (HIF-1α), stromal cell-derived factor-1α (SDF-1α), and vascular endothelial growth factor receptor (KDR). Real-time polymerase chain reaction of CD133, HIF-1α, SDF-1α, and VEGF-1 were also performed. Results: The growth of tumor in EPC group was significantly faster than that in saline solution group (P 0.05). Under fluorescence microscope, GFP-EPCs were strongly expressed in both tumor and bone marrow. EPCs were recruited to the tumor periphery to participate in tumor vasculogenesis. The expression of CD133, HIF-1α, and SDF-1 mRNA in tumor and bone marrow were significantly higher than that in the liver, spleen, and skin (P 0.05). Conclusions: Exogenous EPCs can be recruited to tumor and accelerate tumor growth. Except tumor, bone marrow can also recruit EPCs.