Microbial characteristics in anaerobic membrane bioreactor treating domestic sewage:Effects of HRT and process performance

作     者：Jialing Ni Jiayuan Ji Yu-You Li Kengo Kubota Jialing Ni;Jiayuan Ji;Yu-You Li;Kengo Kubota

作者机构：Department of Civil and Environmental EngineeringGraduate School of EngineeringTohoku University6-6-06 AobaAramakiAoba-ku 980-8579SendaiJapan Department of Frontier Science for Advanced EnvironmentGraduate School of Environmental StudiesTohoku University6-6-06 AobaAramakiAoba-ku 980-8579SendaiJapan 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2022年第34卷第1期

页      面：392-399页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：supported by Grant-in-Aids for JSPS Fellows(KAKENHI Grant nos.JP19J11931 and JP19J12023) Scientific Research(B)(KAKENHI Grant no.JP18H01564) Scientific Research(A)(KAKENHI Grant no.JP19H01160)from Japan Society for the Promotion of Science by Ministry of the Environment,Japan(Low Carbon Technology Research,Development and Demonstration Program:Innovative sewage treatment system for energy saving and energy production,20172019)。 

主　　题：Anaerobic membrane bioreactor Co-occurrence analysis Hydrogenotrophic methanogen HRT Microbial community structure Principal coordinate analysis 

摘      要：Two anaerobic membrane bioreactors(AnMBRs)equipped with different membrane pore size(0.4 or 0.05μm)were operated at 25℃and fed with domestic wastewater.The hydraulic retention time(HRT)of the reactors was shortened.The microbial communities of the two AnMBRs were investigated by 16S rRNA gene amplicon sequencing to see the effects of HRT.The predominant Archaea was an aceticlastic methanogen Methanosaeta.The composition of hydrogenotrophic methanogens changed with the HRTs:the population of Methanobacterium was higher for longer HRTs,whereas the population of unclassified Methanoregulaceae was higher for shorter HRTs.The Anaerolineae,Bacteroidia and Clostridia bacteria were dominant in both of the reactors,with a combined relative abundance of over 55%.The relative abundance of Anaerolineae was proportional to the biogas production performance.The change in the population of hydrogenotrophic methanogens or Anaerolineae can be used as an indicator for process monitoring.The sum of the relative abundance of Anaerolineae and Clostridia fluctuated slightly with changes in the HRT in both AnMBRs when the reactor was stably operated.The co-occurrence analysis revealed the relative abundance of the operational taxonomic units belonging to Anaerolineae and Clostridia was functionally equivalent during the treatment of real domestic sewage.A principal coordination analysis revealed that the changes in the microbial community in each reactor were consistent with the change of HRT.In addition,both the HRT and the stability of the process are important factors for maintaining microbial community structures.