Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection
Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection作者机构：Department of Infection Disease Children＇s Hospital FudanUniversity Shanghai 200032 China
出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)
年 卷 期：2005年第118卷第19期
页 面：1604-1609页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100211[医学-妇产科学] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：ThisresearchwassupportedbyagrantfromtheNationalScience FoundationofChina(No.30271365).
主 题：cytokine gene polymorphism hepatitis B virus infection uterus susceptibility
摘 要：Background The influences of genomic background are confirmed in more diseases. Immunologic tolerance after intrauterine infection of hepatitis B virus is considered to occur in T cells. Cytokines work effectively in eliminating virus by immune system after hepatitis B virus infection. To explore the relationship between cytokines （tumor necrosis factor-α, interferon-γ, interleukin-4 and interleukin-10）, which expressed abnormal quantity in the peripheral blood to intrauterine hepatitis B virus infectious children, gene single nucleotide polymorphism （SNP） and susceptibility to hepatitis B virus intrauterine infection. Methods This is a cross sectional study of molecular clinical epidemiology. The subjects in this study were selected from outpatients of hepatitis B vaccine follow-up special clinics of our hospital in the period. According to intrant criteria, the high risk children of hepatitis B virus （HBV） intrauterine infection were divided into immune failure group （group Ⅰ） ; and immune effective group （group Ⅱ） and non high risk children belonged to the control group. Four gene SNP sites of TNF-α -238, IFN-γ ＋874, IL-4 -590 and IL-10 -1082 were determined by real-time quantitative fluorescent polymerase chain reaction （PCR）. Results The significant differences of TNF-α- 238 A allele frequency were found between group Ⅰand group Ⅱ（X^2 =6. 797, P 〈 0.05 ） and between group Ⅰ and the control group （ X^2 = 9. 513, P 〈 0.05 ）. No evident differences of TNF-α- 238 A were found between group Ⅱand control group （ X^2 = 0. 047, P 〉 0. 05 ） ; the significant differences of IFN-γ ＋ 874 A allele frequency were found between group Ⅰand group Ⅱ（ X^2 = 7. 238, P〈0. 05）, and between group Ⅰand the control group （X^2 =5. 199,P 〈0. 05）. No evident differences were found between groupⅡ and the control group （ X^2 =0. 602 ,P 〉0. 05 ） ; the significant differences of IL-4 -590 C/T allele frequency were not found between groupⅠand group Ⅱ （ X^2 = 0. 632, P 〉 0. 05 ） , also group Ⅰ and the control group （ x^2 = 0. 584, P 〉 0. 05 ）, and the group Ⅱ and the control group （ X^2 = 0. 004, P 〉 0. 05 ） respectively; The significant differences of IL-10 - 1082 G allele frequency were found between group Ⅱand group Ⅰ（x^2 = 10. 359,P 〈0. 001 ）, and between group Ⅱ and the controls （x^2 =35. 418,P 〈0. 001 ）, but the significant differences were not found between group Ⅰ and the control group （ X^2 = 1. 759,P 〉 0. 05 ）. Conclusions This study suggested the possibility that the TNF-α-238 A allele and IFN-γ＋ 874 A allele were associated with HBV intrauterine infection. There was no evident relationship between IL-4 -590 C/T allele SNP and susceptibility to HBV intrauterine infection, but the IL-10 - 1082 G allele was associated with preventive efficacy to HBV intrauterine infection.
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