Serum exosomal microRNA-144-3p:a promising biomarker for monitoring Crohn’s disease

作     者：Peng Chen Shanshan Huang Qiao Yu Kang Chao Ying Wang Gaoshi Zhou Xiaojun Zhuang Zhirong Zeng Minhu Chen Shenghong Zhang 

作者机构：Department of GastroenterologyThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouP.R.China Department of GastroenterologyThe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouP.R.China Department of GastroenterologyThe Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouP.R.China 

出 版 物：《Gastroenterology Report》 (胃肠病学报道（英文）)

年 卷 期：2022年第10卷第1期

页      面：313-321页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China[grant numbers 81630018,82070538,,81870374] Guangdong Science and Technology Department[grant number 2017A030306021] Guangzhou Science and Technology Department[grant number 202002030041] 

主　　题：Crohn’s disease microRNA-144-3p serum biomarker exosome 

摘      要：Background:Crohn’s disease(CD)has a tendency for recurrence and requires adequate monitoring and personalized *** endoscopy is considerably invasive,serum biomarkers are required as alternatives for CD *** this,exosomal microRNAs(miRNAs)may serve as promising *** this study,we aimed to assess the role of serum exosomal microRNA-144-3p(miR-144-3p)as a biomarker for CD ***:We prospectively recruited 154 patients without a history of surgery(Cohort 1)and 75 patients who were to undergo intestinal resection(Cohort 2).Serum samples were collected from Cohort 1 before colonoscopy and from Cohort 2 before surgery and during post-operative colonoscopic *** serum levels of exosomal miR-144-3p were measured using quantitative reverse-transcription polymerase chain reaction(PCR).Correlations between relative exosomal miR-144-3p levels,disease activity,and disease behavior were *** area under the receiver-operating characteristic curve(AUC)was used to assess the predictive value of exosomal miR-144-3p regarding mucosal activity and postoperative ***:A 3.33-fold increase in serum exosomal miR-144-3p levels was recorded in patients with CD compared with those in healthy controls(P0.001).The exosomalmiR-144-3p levels were positively correlated with the simple endoscopic score of CD(q=0.547,P0.001)as well as the Rutgeerts score(q=0.478,P0.001).Elevated exosomalmiR-144-3p levels were correlated with the penetrating disease with high specificity(100%[95%confidence interval,95.1%–100%]).The accuracy of exosomalmiR-144-3p for identifying post-operative recurrence was higher than that of C-reactive protein(CRP)(AUC,0.775 vs 0.639;P0.001).Conclusions:Serum exosomal miR-144-3p is a reliable biomarker of mucosal inflammation and penetrating *** may identify endoscopic CD recurrence after intestinal resection with higher accuracy than CRP testing.