Dietary vitamin D_(3) deprivation suppresses fibroblast growth factor 23 signals by reducing serum phosphorus levels in laying hens

作     者：Jiakun Yan Chong Pan Yanli Liu Xujie Liao Jionghao Chen Yufei Zhu Xinhuo Huang Xiaojun Yang Zhouzheng Ren Jiakun Yan;Chong Pan;Yanli Liu;Xujie Liao;Jionghao Chen;Yufei Zhu;Xinhuo Huang;Xiaojun Yang;Zhouzheng Ren

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxiChina Nano Vitamin Engineering Research Center of Shaanxi ProvinceXi'anChina 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第9卷第2期

页      面：23-30页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：the National Natural Science Foundation of China(31902175) Shaanxi Provincial Key Research and Development Program(2019NY-077) Shaanxi Feed Engineering Technology Research Center(2019HBGC-16) the Program for Shaanxi Science&Technology from Shaanxi Provincial Science and Technology Department(2021TD-30) 

主　　题：Dietary vitamin D_(3) Fibroblast growth factor 23 Fibroblast growth factor receptor KLOTHO Laying hen 

摘      要：The present study was carried out to evaluate the effect of dietary supplemental vitamin D_(3) on fibroblast growth factor 23(FGF23)signals as well as phosphorus homeostasis and metabolism in laying *** 40-week-old Hy-Line Brown layers were randomly assigned into 2 treatments:1)vitamin D_(3) restriction group(n=7)fed 0 IU/kg vitamin D_(3) diet,and 2)regular vitamin D_(3) group(n=7)fed 1,600 IU/kg vitamin D_(3) *** study lasted for 21 *** parameters,phosphorus and calcium excretion status,and tissue expressions of type II sodium-phosphate co-transporters(NPt2),FGF23 signals and vitamin D_(3) metabolic regulators were *** fed the vitamin D_(3) restricted diet had decreased serum phosphorus levels(by 31.3%,P=0.028)when compared to those fed regular vitamin D_(3) *** response to the decreased serum phosphorus,the vitamin D_(3) restricted laying hens exhibited:1)suppressed kidney expressions of 25-hydroxyvitamin D 1-a-hydroxylase(CYP27B1,by 52.8%,P=0.036)and 1,25-dihydroxyvitamin D 24-hydroxylase(CYP24A1,by 99.4%,P=0.032);2)suppressed serum levels of FGF23(by 14.6%,P=0.048)and increased serum alkaline phosphatase level(by 414.1%,P=0.012);3)decreased calvaria mRNA expressions of fibroblast growth factor receptors(FGFR1,by 85.2%,P=0.003,FGFR2,by 89.4%,P=0.014,FGFR3,by 88.8%,P=0.017,FGFR4,by 89.6%,P=0.030);4)decreased kidney mRNA expressions of FGFR1(by 65.5%,P=0.021),FGFR4(by 66.0%,P=0.050)and KLOTHO(by 68.8%,P=0.038);5)decreased kidney protein expression of type 2a sodium-phosphorus co-transporters(by 54.3%,P=0.039);and 6)increased percent excreta calcium(by 26.9%,P=0.002).In conclusion,the deprivation of dietary vitamin D_(3) decreased FGF23 signals in laying hens by reducing serum FGF23 level and suppressing calvaria and kidney mRNA expressions of FGF23 receptors.